Changes in neuronal activity across the mouse ventromedial nucleus of the hypothalamus in response to low glucose: Evaluation using an extracellular multi-electrode array approach.
Changes in neuronal activity across the mouse ventromedial nucleus of the hypothalamus in response to low glucose: Evaluation using an extracellular multi-electrode array approach.
复制标题
小鼠下丘脑腹内侧核神经元活动的变化响应低葡萄糖：使用细胞外多电极阵列方法进行评估。
DOI：
10.1111/jne.12824
复制
发表时间：
2020
影响因子：
3.2
通讯作者：
Hanna L
中科院分区：
文献类型：
--
作者：
Hanna L
下丘脑腹内侧核（VMN）参与维持全身葡萄糖稳态。啮齿动物脑切片的神经生理学研究已经确定了VMN葡萄糖敏感神经元的群体：葡萄糖兴奋（GE）神经元，即响应于葡萄糖浓度增加而增加其放电频率的细胞，以及葡萄糖抑制（GI）神经元，其显示响应于葡萄糖浓度增加而降低的放电频率。迄今为止，大多数表征啮齿动物VMN葡萄糖敏感神经元的切片电生理学研究都使用了膜片钳技术。多电极阵列（MEA）是一种最先进的电生理工具，能够同时记录多个电极部位（通道）上许多细胞的电活动。我们使用穿孔MEA（pMEA）系统来评估小鼠VMN区域背腹侧范围内的电活动变化，以响应葡萄糖浓度的变化。由于内在的（即直接突触后感知）和外在的（即突触前调制）葡萄糖感觉没有区别，我们使用的术语“GE/突触前兴奋的增加（PER）”和“GI/突触前兴奋的减少（PED）”在本研究中描述的小鼠VMN细胞外葡萄糖的变化的反应。我们观察到15%-60%的通道是GE/PER，而2%-7%是GI/PED通道。在VMN的背内侧部分（DM-VMN）内，与VMN的腹外侧部分（VL-VMN）相比，GE/PER通道明显更多。然而，在2.5 mmol l-1葡萄糖中，VL-VMN内的GE/PER通道显示出比DM-VMN GE/PER通道显著更高的基础放电速率。胃肠道/PED通道的分布没有显着差异，观察VMN子区域之间。本研究的结果证明了pMEA方法用于评价小鼠VMN的葡萄糖响应性的实用性。pMEA研究可用于通过检查脑核团电活动的群体水平变化来完善我们对其他神经内分泌系统的理解，从而提供关键的功能神经解剖信息，以补充和告知单细胞神经生理学研究的设计。
The hypothalamic ventromedial nucleus (VMN) is involved in maintaining systemic glucose homeostasis. Neurophysiological studies in rodent brain slices have identified populations of VMN glucose‐sensing neurones: glucose‐excited (GE) neurones, cells which increased their firing rate in response to increases in glucose concentration, and glucose‐inhibited (GI) neurones, which show a reduced firing frequency in response to increasing glucose concentrations. To date, most slice electrophysiological studies characterising VMN glucose‐sensing neurones in rodents have utilised the patch clamp technique. Multi‐electrode arrays (MEAs) are a state‐of‐the‐art electrophysiological tool enabling the electrical activity of many cells to be recorded across multiple electrode sites (channels) simultaneously. We used a perforated MEA (pMEA) system to evaluate electrical activity changes across the dorsal‐ventral extent of the mouse VMN region in response to alterations in glucose concentration. Because intrinsic (ie, direct postsynaptic sensing) and extrinsic (ie, presynaptically modulated) glucosensation were not discriminated, we use the terminology ‘GE/presynaptically excited by an increase (PER)’ and ‘GI/presynaptically excited by a decrease (PED)’ in the present study to describe responsiveness to changes in extracellular glucose across the mouse VMN. We observed that 15%‐60% of channels were GE/PER, whereas 2%‐7% were GI/PED channels. Within the dorsomedial portion of the VMN (DM‐VMN), significantly more channels were GE/PER compared to the ventrolateral portion of the VMN (VL‐VMN). However, GE/PER channels within the VL‐VMN showed a significantly higher basal firing rate in 2.5 mmol l‐1glucose than DM‐VMN GE/PER channels. No significant difference in the distribution of GI/PED channels was observed between the VMN subregions. The results of the present study demonstrate the utility of the pMEA approach for evaluating glucose responsivity across the mouse VMN. pMEA studies could be used to refine our understanding of other neuroendocrine systems by examining population level changes in electrical activity across brain nuclei, thus providing key functional neuroanatomical information to complement and inform the design of single‐cell neurophysiological studies.