Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents
Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents
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DOI：
10.1590/s1984-82502009000200008
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发表时间：
2009-06-01
影响因子：
1.3
通讯作者：
Penna, Thereza Christina Vessoni
中科院分区：
文献类型：
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作者：
Mazzola, Priscila Gava;Jozala, Angela Faustino;Penna, Thereza Christina Vessoni
由于医院和托儿所感染爆发的数量不断增加，因此必须建立一个卫生计划，以表明以最有效的方式选择适当的化学制剂。验证去污和消毒的有效性是一项重要且经常具有挑战性的任务。为了研究和比较所选微生物的行为，将它们提交到最小抑菌浓度（MIC）。使细菌总数减少超过6log 10的MIC区间为：季铵化合物（QAC）59至156 mg/L-氯己定63至10000 mg/L;戊二醛1375至3250 mg/L;甲醛39至246 mg/L;乙醇43750至87500 mg/L;聚乙烯吡咯烷酮络合物中的1250至6250 mg/L碘，150至4491 mg/L氯释放剂（CRA）和469至2500 mg/L氢氧化物。洗必泰对孢子萌发无抑制作用。A.醋酸钙杆菌对大多数药物具有耐药性，其次是E. cloxacin和S. marcescens。
Due to the growing number Of Outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is ail important and often challenging task. In order to study and compare the behavior of selected microorganisms, they Were Submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria Populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds (QACs)- 63 to 10000 mg/L of chlorhexidine; 1375 to 3250 mg/L of glutaraldehyde; 39 to 246 mg/L of formaldehyde; 43750 to 87500 mg/L of ethanol; 1250 to 6250 mg/L of iodine in polyvinyl-pyrolidone complexes, 150 to 4491 mg/L of chlorine-releasing-agents (CRAs) and 469 to 2500 mg/L of hydrogen pet-oxide. Chlorhexidine showed non inhibitory activity over germinating spores. A. calcoaceticus showed resistance to the majority of the agents tested, followed by E. cloacae and S. marcescens.