INVESTIGATIONS INTO THE IMMUNOTOXICITY AND ALLERGIC POTENTIAL INDUCED BY TOPICAL APPLICATION OF N-BUTYLBENZENESULFONAMIDE (NBBS) IN A MURINE MODEL
INVESTIGATIONS INTO THE IMMUNOTOXICITY AND ALLERGIC POTENTIAL INDUCED BY TOPICAL APPLICATION OF N-BUTYLBENZENESULFONAMIDE (NBBS) IN A MURINE MODEL
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DOI：
10.1080/15287394.2015.1056898
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发表时间：
2015-09-02
影响因子：
2.6
通讯作者：
Anderson, Stacey E.
中科院分区：
文献类型：
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作者：
Marrocco, Antonella;Meade, B. Jean;Anderson, Stacey E.
N-丁基苯磺酰胺（NBBS）是一种常用的增塑剂，存在于许多产品中。由于其广泛使用，缺乏足够的毒理学数据，以及基于结构警报的存在而怀疑其具有毒性，因此被提名为国家毒理学计划进行全面的毒理学测试。本研究的目的是使用小鼠模型评价皮肤暴露于NBBS后发生超敏反应和免疫抑制的可能性。NBBS在联合刺激性/局部淋巴结试验（LLNA）中检测为阴性，将其归类为无刺激性和无致敏性。为了估计NBBS的免疫抑制潜力，进行了评估免疫毒性的试验，包括使用空斑形成细胞（PFC）试验和免疫细胞表型分析的免疫球蛋白（IG）M对T细胞依赖性抗原绵羊红细胞（SRBC）的反应。在用NBBS处理28天后，暴露于最低浓度（25%NBBS）的小鼠显示脾中产生IgM的B细胞显著增加。淋巴结中的免疫细胞标志物未发现明显变化。与体重相比，皮肤接触所有浓度的NBBS后，观察到肾脏和肝脏重量显著升高。这些结果表明，皮肤暴露于NBBS（肝脏和肾脏毒性除外）在小鼠模型中未明显诱导免疫毒性。
N-Butylbenzene sulfonamide (NBBS) is a commonly used plasticizer found in numerous products. Due to its extensive use, lack of adequate toxicological data, and suspicion of toxicity based on the presence of structural alerts, it was nominated to the National Toxicology Program for comprehensive toxicological testing. The purpose of this study was to evaluate the potential for hypersensitivity and immune suppression following dermal exposure to NBBS using a murine model. NBBS tested negative in a combined irritancy/local lymph node assay (LLNA), classifying it as nonirritating and nonsensitizing. To estimate the immunosuppressive potential of NBBS, assays that assessed immunotoxicity were performed, including the immumnoglobulin (Ig) M response to T-cell-dependent antigen sheep red blood cells (SRBC), using the plaque-forming cell (PFC) assay and immune cell phenotyping. After a 28-d treatment with NBBS, mice exposed to the lowest concentration (25% NBBS) showed a significant increase in IgM-producing B cells in the spleen. No marked changes were identified in immune cell markers in the lymph node. In contrast to body weight, a significant elevation in kidney and liver weight was observed following dermal exposure to all concentrations of NBBS. These results demonstrate that dermal exposure to NBBS, other than liver and kidney toxicity, did not apparently induce immunotoxicity in a murine model.