Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research
Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research
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DOI：
10.1186/1742-4682-3-10
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Cooper, Stephen
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文献类型：
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作者：
Cooper, Stephen
背景：了解细胞周期中生长的两种方法是单细胞研究和细胞培养研究，其中单细胞研究测量单个细胞在细胞周期中的生长，细胞培养研究将大量细胞作为一个聚集体在细胞周期中的生长进行分析。米奇森提出，单细胞研究更适合于理解细胞周期中的细胞生长，因为它们显示了细胞生长模式的变化，应该优先于培养研究。具体地说，米奇森认为，人们可以通过显微镜观察分裂周期中的单个细胞来收集细胞生长模式。与米奇森的观点相反，这里的论点是，在单细胞研究中找不到细胞生长的生物学规律。结果：提出并讨论了细胞周期分析的目的或目标。这些观点被应用于支持在细胞周期中作为一种可能的生长模式的线性增长的支持者和在细胞周期中支持指数增长的支持者之间的争论。在细胞周期分析方面，比较了微分(脉冲)实验和积分(单细胞)实验，得出结论：为了区分线性和指数生长模式，脉冲标记方法比显微镜检查细胞生长更可取。更重要的是，集合实验比单细胞研究更受欢迎。结论：指数增长的逻辑一致性-整合并解释了生物化学、细胞生物学和严格的实验分析-导致了线性增长的提议是实验扰动和测量限制的结果。有人提出，细胞周期中细胞生长的普遍模式是指数型的。
Background: Two approaches to understanding growth during the cell cycle are single-cell studies, where growth during the cell cycle of a single cell is measured, and cell-culture studies, where growth during the cell cycle of a large number of cells as an aggregate is analyzed. Mitchison has proposed that single-cell studies, because they show variations in cell growth patterns, are more suitable for understanding cell growth during the cell cycle, and should be preferred over culture studies. Specifically, Mitchison argues that one can glean the cellular growth pattern by microscopically observing single cells during the division cycle. In contrast to Mitchison's viewpoint, it is argued here that the biological laws underlying cell growth are not to be found in single-cell studies. The cellular growth law can and should be understood by studying cells as an aggregate.Results: The purpose or objective of cell cycle analysis is presented and discussed. These ideas are applied to the controversy between proponents of linear growth as a possible growth pattern during the cell cycle and the proponents of exponential growth during the cell cycle. Differential (pulse) and integral (single cell) experiments are compared with regard to cell cycle analysis and it is concluded that pulse-labeling approaches are preferred over microscopic examination of cell growth for distinguishing between linear and exponential growth patterns. Even more to the point, aggregate experiments are to be preferred to single-cell studies.Conclusion: The logical consistency of exponential growth - integrating and accounting for biochemistry, cell biology, and rigorous experimental analysis - leads to the conclusion that proposals of linear growth are the result of experimental perturbations and measurement limitations. It is proposed that the universal pattern of cell growth during the cell cycle is exponential.