Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA.
Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA.
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176 kb 的小鼠 T 复合体 DNA 中有 11 个密集聚集的基因，其中 6 个是新基因。
DOI：
10.1101/gr.10.7.916
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发表时间：
2000
期刊：
影响因子：
7
通讯作者：
Ko,MS
中科院分区：
文献类型：
--
作者：
Kargul,GJ;Nagaraja,R;Shimada,T;Grahovac,MJ;Lim,MK;Nakashima,H;Waeltz,P;Ma,P;Chen,E;Schlessinger,D;Ko,MS
小鼠复合体的靶向测序始于176 kb的富含基因的BAC，该BAC在高度重复区域的倒位2/3中定位有六个基于PCR的标记。该序列包含11个主要以cDNA形式从早期胚胎收集物中回收的基因，包括Igfals（先前位于17号染色体上）、Nubp 2（一种完全表征的基因）、Jsap 1（一种JNK结合蛋白）、Rsp 29（大鼠基因的小鼠同源物）、Ndk 3（一种核苷二磷酸激酶）和另外6个功能未知的推定基因。50%的GC含量，75%的DNA转录，和一个基因/16.0 kb（平均），该地区可能有资格作为一个最密集的基因片段在小鼠基因组中，并提供了剂量敏感的表型和小鼠胚胎致死映射到附近的候选人。[The本文中描述的序列数据已提交至GenBank数据库，登录号为AF 220294。
Targeted sequencing of the mouset-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, includingIgfals(previously placed on chromosome 17),Nubp2(a fully characterized gene),Jsap1(a JNK-binding protein),Rsp29(the mouse homologue of the rat gene),Ndk3(a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.[The sequence data described in this paper have been submitted to the GenBank data library under accession no. AF220294.]