OSCILLATIONS OF CA++ CONCENTRATION DURING THE CELL-DIFFERENTIATION OF DICTYOSTELIUM-DISCOIDEUM - THEIR RELATION TO OSCILLATIONS IN CYCLIC-AMP AND OTHER COMPONENTS
OSCILLATIONS OF CA++ CONCENTRATION DURING THE CELL-DIFFERENTIATION OF DICTYOSTELIUM-DISCOIDEUM - THEIR RELATION TO OSCILLATIONS IN CYCLIC-AMP AND OTHER COMPONENTS
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DOI：
10.1111/j.1432-0436.1986.tb00387.x
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发表时间：
1986-01-01
期刊：
影响因子：
2.9
通讯作者：
WURSTER, B
中科院分区：
文献类型：
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作者：
BUMANN, J;MALCHOW, D;WURSTER, B
周期性的环磷酸腺苷脉冲控制盘基网柄菌的细胞聚集和分化。这些细胞聚集和分化所需的另一种成分似乎是细胞外 Ca++。研究了分化细胞悬液中细胞外 Ca++ 浓度的振荡。我们观察到尖峰状和正弦 Ca++ 振荡先于正弦振荡，并且从尖峰状 Ca++ 振荡过渡到正弦 Ca++ 振荡时没有发生相变。尖峰形和正弦 Ca++ 振荡与以下振荡有关：(1) 细胞的环 AMP 和环 GMP 含量；(2) 细胞的光散射特性；(3) 细胞外 pH 值。观察到尖峰状 Ca++ 振荡和环 AMP 振荡。细胞外 Ca++ 浓度的最小值落后于环 AMP 浓度的最大值约 30 秒。正弦 Ca++ 振荡不伴随可测量的循环 AMP 振荡。正弦 Ca++ 振荡的振幅小于尖峰状 Ca++ 振荡的振幅。当跳过一个循环 AMP 尖峰时，观察到小幅度的 Ca++ 振荡（而不是尖峰状振荡）。我们的结果为存在小幅度的正弦循环 AMP 独立的 Ca++ 振荡提供了证据，并且它们还表明尖峰形 Ca++ 振荡可能叠加在这种小幅度振荡上。当盘状 D. discoideum 细胞产生环 AMP 尖峰时，会诱导吸收额外的 Ca++，从而导致大幅度的 Ca++ 振荡。
Periodic cyclic-AMP pulses control the cell aggregation and differentiation of Dictyostelium discoideum. Another component required for the aggregation and differentiation of these cells appears to be extracellular Ca++. Oscillations in extracellular Ca++ concentration were investigated in suspensions of differentiating cells. We observed spike-shaped and sinusoidal Ca++ oscillations preceded sinusoidal oscillations, and no phase change occurred at the transition from spike-shaped to sinusoidal Ca++ oscillations. Spike-shaped and sinusoidal Ca++ oscillations were related to oscillations in (1) the cyclic-AMP and cyclic-GMP content of cells (2) the light-scattering properties of cells, and (3) the extracellular pH. Spike-shaped Ca++ oscillations were observed together with cyclic-AMP oscillations. The minima of the extracellular Ca++ concentration trailed the maxima of the cyclic-AMP concentration by about 30 s. Sinusoidal Ca++ oscillations were not accompanied by measureable cyclic-AMP oscillations. The amplitudes of the sinusoidal Ca++ oscillations were smaller than those of the spike-shaped Ca++ oscillations. A Ca++ oscillation of small amplitude (instead of a spike-shaped oscillation) was observed when one cyclic-AMP spike was skipped. Our results provide evidence for the existence of a sinusoidal cyclic-AMP-independent Ca++ oscillation of small amplitude, and they also suggest that spike-shaped Ca++ oscillations may be superimposed on such small-amplitude oscillations. When D. discoideum cells produce cyclic-AMP spikes, the uptake of additional Ca++ is induced resulting in Ca++ oscillations of a large amplitude.