Methods for Isolating and Analyzing Physiological Hyaluronan: A Review.
Methods for Isolating and Analyzing Physiological Hyaluronan: A Review.
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DOI：
10.1152/ajpcell.00019.2022
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发表时间：
2022-02
期刊：
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通讯作者：
Felipe Rivas;Dorothea Erxleben;I. Smith;Elaheh Rahbar;Paul J DeAngelis;M. Cowman;A. Hall
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文献类型：
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作者：
Felipe Rivas;Dorothea Erxleben;I. Smith;Elaheh Rahbar;Paul J DeAngelis;M. Cowman;A. Hall
碳水化合物透明质酸（或透明质酸，HA）存在于所有人体组织和生物流体中，在健康和疾病中具有广泛的功能，这取决于其丰度和大小。因此，生理样品中的透明质酸评价具有显著的转化潜力。虽然用于探测其他生物分子（如蛋白质和核酸）的分析工具和技术已成为生物化学中的标准方法，但用于研究透明质酸的分析工具和技术还不太成熟。在这篇综述中，我们调查的方法相关的评估天然透明质酸在生物标本中，包括协议从生物基质和技术，用于确定其浓度和分子量。
The carbohydrate hyaluronan (or hyaluronic acid, HA) is found in all human tissues and biofluids where it has wide-ranging functions in health and disease that are dictated by both its abundance and size. Consequently, hyaluronan evaluation in physiological samples has significant translational potential. While the analytical tools and techniques for probing other biomolecules like proteins and nucleic acids have become standard approaches in biochemistry, those available for investigating hyaluronan are less well-established. In this review, we survey methods related to the assessment of native hyaluronan in biological specimens, including protocols for separating it from biological matrices and technologies for determining its concentration and molecular weight.