Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates
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DOI：
10.1163/156856199x00190
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发表时间：
1999-01-01
影响因子：
2.3
通讯作者：
Oliveira, R
中科院分区：
文献类型：
--
作者：
Teixeira, P;Oliveira, R
研究了产碱杆菌对几种高分子材料的粘附性。由于微生物和基质材料表面的性质是粘附过程中的重要因素，因此还确定了诸如电动势和疏水性的特性，并将其与细菌细胞粘附到固体表面的能力相关联。使用的基材是高密度聚乙烯（HDPE）、聚丙烯（PP）、聚氯乙烯（PVC）和聚甲基丙烯酸甲酯（PMMA）。通过测量电泳迁移率来确定细胞和基板的电动势，并且通过测量接触角来确定疏水性。研究的所有底物以及细菌菌株都具有负zeta电位，这意味着粘附不是由静电相互作用介导的。就疏水性而言，PP是最疏水的材料，PMMA是最不疏水的，而HDPE和PVC呈现中间行为。由于细菌细胞是亲水性的，粘附有利于PP;因此，这种基质材料似乎是促进更强粘附和最稳定的生物膜的发展的材料，其用作生物质载体在反相流化床反应器中。这一点通过粘附性测试的结果得到了证实。以这种方式，粘附似乎由疏水相互作用主导。
The adhesion of Alcaligenes denitrificans to several polymeric materials was investigated. As the nature of the surfaces of the micro-organisms and the substrate materials is an important factor in the adhesion process, characteristics such as the electrokinetic potential and hydrophobicity were also determined and correlated with the capacity of bacterial cells to adhere to solid surfaces. The substrates used were high-density polyethylene (HDPE), polypropylene (PP), poly(vinyl chloride) (PVC), and poly(methyl methacrylate) (PMMA). The electrokinetic potential of the cells and the substrates was determined by measurements of electrophoretic mobility and the hydrophobicity was determined by contact angle measurements. All the substrates studied as well as the bacterial strain have a negative zeta potential, which means that adhesion is not mediated by electrostatic interactions. As far as hydrophobicity is concerned, PP is the most hydrophobic material, PMMA is the least hydrophobic, whereas HDPE and PVC present an intermediate behavior. As bacteria cells are hydrophilic, adhesion is favored to PP; therefore, this substrate material seems to be the one that promotes a stronger adhesion and the development of the most stable biofilm for use as a biomass carrier in denitrifying inverse fluidized bed reactors. This was confirmed by the results of adhesion tests. In this way, adhesion seems to be dominated by hydrophobic interactions.