Comparison of sample preservation methods for clinical trace element analysis by inductively coupled plasma mass spectrometry
Comparison of sample preservation methods for clinical trace element analysis by inductively coupled plasma mass spectrometry
复制标题
DOI：
10.1309/l241wuer8831glwb
复制
发表时间：
2005-04-01
影响因子：
3.5
通讯作者：
McMillin, GA
中科院分区：
文献类型：
--
作者：
Bornhorst, JA;Hunt, JW;McMillin, GA
研究了化学添加剂和储存温度对电感耦合等离子体质谱法（ICP-MS）测定尿中16种微量元素的影响。24小时尿样补充了元素浓度。将含有1/4种化学添加剂的等分试样在3个不同温度下储存在密封的聚丙烯容器中。在储存1、2、8和65天后，通过ICP-MS测定所得样品的元素浓度。在样本制备后8小时内测量的初始元素浓度与预期浓度一致（铝除外）。对于大多数元素，保存和储存条件在整个实验中产生一致的测量浓度。值得注意的例外是铝（随时间推移而普遍上升）和汞（随时间推移而普遍下降）。添加硼酸和焦硫酸钾导致样品污染;元素污染对两者都是浓度依赖性的。虽然在实验过程中观察到很少的微生物污染，但建议冷藏样品以减少非无菌样品中的细菌生长。根据这些结果，不使用化学添加剂的冷藏尿液储存是许多微量元素的ICP-MS分析的有效保存方法。
The effects of chemical additives and storage temperatures on measurement of 16 trace elements in urine by inductively coupled plasma mass spectrometry (ICP-MS) were evaluated. A 24-hour urine specimen was supplemented with concentrations of the elements. Aliquots containing I of 4 chemical additives were stored at 3 different temperatures in sealed polypropylene containers. Elemental concentrations were determined by ICP-MS for the resulting samples after 1, 2, 8, and 65 days of storage. Initial element concentrations measured within 8 hours of specimen preparation were consistent with expected concentrations (except for aluminum). For most elements, preservation and storage conditions yielded consistent measured concentrations throughout the experiment. Notable exceptions were for aluminum (general rise over time) and mercury (general decrease over time). Adding boric acid and potassium pyrosulfate resulted in sample contamination; elemental contamination was concentration-dependent for both. Although little microbial contamination was observed during the experiment, refrigeration of samples is recommended to curtail bacterial growth in nonsterile specimens. In light of these results, refrigerated urine storage without the use of chemical additives is an effective preservation method for ICP-MS analysis of many trace elements.