The presence of a soluble interphotoreceptor retinol‐binding protein (IRBP) in the retinal interphotoreceptor space
The presence of a soluble interphotoreceptor retinol‐binding protein (IRBP) in the retinal interphotoreceptor space
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视网膜感光器间空间中存在可溶性感光器间视黄醇结合蛋白（IRBP）
DOI：
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发表时间：
1983
影响因子：
5.6
通讯作者：
G. Chader
中科院分区：
文献类型：
--
作者：
B. A. Pfeffer;B. Wiggert;L. Lee;B. Zonnenberg;D. Newsome;G. Chader
一种新的，温和的技术已经开发用于冲洗视网膜感光细胞间隙（IPS），以获得细胞外基质（ECM）的可溶性成分。用这种方法，我们确定了猴IPS的主要可溶性成分是一种146，000 Mr的糖蛋白，它与[~ 3 H]视黄醇结合，在7S蔗糖梯度上沉淀，在非变性凝胶电泳上Rf为0.42。使用尺寸排阻高效液相色谱法计算天然蛋白质的表观分子量为250，000道尔顿。与之前的研究相反，在IPS冲洗液中未观察到15，000-道尔顿细胞视黄醇结合蛋白（CRBP）或33，000-道尔顿细胞视黄醇脱结合蛋白（CRALBP），表明这些蛋白可能不参与视网膜和色素上皮（PE）之间的视黄醇转运。在含有可溶性细胞内蛋白质以及细胞外成分的视网膜匀浆的上清液部分中，146，000 Mr蛋白质与93，000 Mr蛋白质密切相关，可以在SDS凝胶电泳上分离;在IPS洗涤液中未发现93，000 Mr蛋白质。146，000 Mr感光细胞间视黄醇结合蛋白（IRBP）可能在IPS的细胞外视黄醇转运中发挥作用。
A new, gentle technique has been developed for washing of the retinal interphotoreceptor space (IPS) to obtain soluble components of the extracellular matrix (ECM). Using this method, we have determined that the major soluble coustituent of monkey IPS is a 146,000 Mr glycoprotein, which binds [3H]retinol, sediments on sucrose gradients at 7S and has an Rf of 0.42 on native gel electrophoresis. Using size‐exclusion high performance liquid chromatography, the apparent molecular weight of the native protein was calculated to be 250,000 daltons. In contrast to previous studies, no 15,000‐dalton cellular retinol‐binding protein (CRBP) or 33,000‐dalton cellular retinaldehydebinding protein (CRALBP) was observed in the IPS wash, indicating that these proteins are probably not involved in retinol transport between retina and pigment epithelium (PE). In the supernatant fraction of retinal homogenates that contains soluble intracellular proteins as well as extracellular constituents, the 146,000 Mr protein was closely associated with a 93,000 Mr protein that could be separated on SDS‐gel electrophoresis; the 93,000 Mr protein was not found in the IPS wash. The 146,000 Mr interphotoreceptor retinol‐binding protein (IRBP) may function in extracellular retinol transport in the IPS.
鉴定与来自牛视网膜和视网膜色素上皮的三种细胞类视黄醇结合蛋白相关的内源性类视黄醇。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Saari,JC;Bredberg,L;Garwin,GG
通讯作者：
Garwin,GG