Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p
Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p
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DOI：
10.1038/ncb789
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发表时间：
2002-05
影响因子：
21.3
通讯作者：
H. Murakami;S. Yanow;D. Griffiths;M. Nakanishi;P. Nurse
中科院分区：
文献类型：
--
作者：
H. Murakami;S. Yanow;D. Griffiths;M. Nakanishi;P. Nurse
当DNA损伤或DNA复制不完全时，S期和DNA损伤检查点控制阻断有丝分裂的开始。有人提出，受损或不完全复制的DNA会产生检查点控制途径所感知的结构，尽管对所涉及的结构和机制知之甚少。在这里，我们表明，DNA复制起始蛋白Orp 1 p和Cdc 18 p，需要诱导和维持的S-期检查点inSchizosophyomycespombe。DNA复制结构的存在与Cds 1 p检查点蛋白激酶和S期检查点通路的激活相关。相反，DNA损伤途径的诱导不依赖于Orp 1 p或Cdc 18 p。我们建议，未解决的复制叉的存在，连同Orp 1 p和Cdc 18 p，激活Cds 1 p依赖的S期检查点是必要的。
S-phase and DNA damage checkpoint controls block the onset of mitosis when DNA is damaged or DNA replication is incomplete,,. It has been proposed that damaged or incompletely replicated DNA generates structures that are sensed by the checkpoint control pathway,, although little is known about the structures and mechanisms involved. Here, we show that the DNA replication initiation proteins Orp1p,and Cdc18p,are required to induce and maintain the S-phase checkpoint inSchizosaccharomyces pombe. The presence of DNA replication structures correlates with activation of the Cds1p checkpoint protein kinase and the S-phase checkpoint pathway. By contrast, induction of the DNA damage pathway is not dependent on Orp1p or Cdc18p. We propose that the presence of unresolved replication forks, together with Orp1p and Cdc18p, are necessary to activate the Cds1p-dependent S-phase checkpoint.