Putrescine Oxidase of Micrococcus rubens
Putrescine Oxidase of Micrococcus rubens
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红微球菌腐胺氧化酶
DOI：
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发表时间：
1965
期刊：
影响因子：
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通讯作者：
K. Ogata
中科院分区：
文献类型：
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作者：
H. Yamada;A. Tanaka;K. Ogata
先生道：各种细菌细胞对天然二胺的氧化作用已经报道过。3）腐胺被二胺氧化酶氧化脱氨。然而，细菌二胺氧化酶的性质仍然未知。在研究微生物代谢胺的过程中，我们发现红色微球菌（Micrococcus rubens）细胞中存在显著的“腐胺氧化酶”。本文讨论腐胺氧化酶的性质。在含1%肉膏、1%蛋白胨、0.5%NaCl和0.5%酵母膏的培养基（pH7.0）中连续振荡培养48小时。用超声波振荡法从菌体中提取酶，经鱼精蛋白沉淀、硫酸铵分级（pH7.0时饱和度为50 × 60%）和DEAE-纤维素层析纯化30 × 35倍。这种酶制剂能迅速氧化腐胺。尸胺和亚精胺被氧化，但氧化速率相当低。其它单胺、二胺和多胺根本不被氧化。结果示于表I中。腐胺如下氧化：腐胺+02的TM 1-吡咯啉+ NH3+ H2 O2。吡咯啉和氨以化学计量的量形成。通过Holmstedt、Larsson和Tham的比色法（5）和表I测定吡咯啉。红色微球菌腐殖酸氧化酶氧化各种胺的相对速率
Sir: The oxidation of natural diamines by cells of various bacteria has already been report ed.1•`3) It has been assumed that putrescine is oxidatively deaminated by a diamine oxi dase. However, the properties of the bacterial diamine oxidase remain unknown. In the course of investigations on the metabolism of amines by microorganisms, we have found that "putrescine oxidase" occurs remarkably in the cells of Micrococcus rubens. This communication deals with the properties of the putrescine oxidase. Mirococcus rubens (IFO 3768) was grown for 48 hours in a medium (pH 7.0) contain ing 1% meat extract, 1% peptone, 0.5% NaCl and 0.5% yeast extract, with continuous shak ing. The enzyme was extracted from the cells by ultrasonic oscillation and purified 30 •` 35 fold by protamine precipitation, ammo nium sulfate fractionation (50•`60% saturation at pH 7.0) and DEAE-cellulose chromato graphy. This enzyme preparation oxidized putrescine rapidly. Cadaverine and spermidine were oxidized but in considerably lower rates. The other monoamines, diamines and poly amine were not oxidized at all. The result is shown in Table I. Putrescine was oxidized as follows: Putrescine +02‡TM1-Pyrroline+ NH3+H202. Pyrroline and ammonia were formed in stoichiometric amounts. Pyrroline was determined by the colorimetric method of Holmstedt, Larsson and Tham,5) and amTABLE I. RELATIVE RATES OF OXIDATION OF VARIOUS AMINES BY PUTRESCINE OXIDASE OF Micrococcus rubens