Biochemical response of soybean roots to Fusarium solani f. sp glycines infection
Biochemical response of soybean roots to Fusarium solani f. sp glycines infection
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DOI：
10.2135/cropsci2004.0819
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发表时间：
2004-05-01
期刊：
影响因子：
2.3
通讯作者：
Widhohn, JM
中科院分区：
文献类型：
--
作者：
Lozovaya, VV;Lygin, AV;Widhohn, JM
土传真菌Fusarium solani（Mart.）SACC. F.大豆（Glycine max（L.）Merr.]猝死综合征（Sudden Death Syndrome，SDS）是该病的根源和病因。通过比较两个部分抗性基因型（“PI 520733”和“PI 567374”）和感病基因型（“Spencer”）接种和未接种FSG的根，研究了大豆根对FSG感染的生化反应。苯丙氨酸解氨酶（PAL），在苯丙烷类生物合成途径中的第一个酶的活性，在所有三个基因型接种根增加。部分抗性品种PI 520733和PI 567374接种根中的植物抗毒素glyceollin增加到比感病品种Spencer更高的水平。酚类代谢的变化是本地化的病变含区域的根，而不是在新的部分下生长的FSG接种。大豆抗毒素前体大豆苷元（4 '，7-二羟基异黄酮）及其结合物与根组织中大豆抗毒素水平之间没有明显的相关性;然而，大豆抗毒素水平仅在部分抗性品系的接种植物的根中增加，即使在易感对照中组成型大豆抗毒素水平更高。在马铃薯葡萄糖琼脂培养基上，随着大豆抗毒素浓度的增加，FSG的生长受到抑制。木质素合成的诱导被发现在所有三个线的接种根中，观察到的部分抗性基因型，特别是PI 567374根中的木质化率最高。这些研究首次表明，FSG接种大豆根在土壤中诱导的苯丙素途径合成异黄酮，植物抗毒素glyceollin，和木质素，表明这些化合物可能参与部分抗性反应。
The soil-borne fungus Fusarium solani (Mart.) Sacc. f. sp. glycines (FSG) infects soybean [Glycine max (L.) Merr.] roots and causes the disease sudden death syndrome (SDS). The biochemical response of soybean roots to FSG infection, which has not been studied before, was investigated by comparing FSG-inoculated and noninoculated roots of two partially resistant ('PI 520733' and 'PI 567374') and susceptible ('Spencer') genotypes. Activity of phenylalanine ammonia-lyase (PAL), the first enzyme in the phenylpropanoid biosynthetic pathway, was increased in inoculated roots of all three genotypes. The phytoalexin glyceollin increased to much higher levels in inoculated roots of the partially resistant cultivars PI 520733 and PI 567374 than in the susceptible Spencer. The changes in phenolic metabolism were localized in lesion-containing areas of roots rather than in the new portion growing under the FSG inoculum. No clear correlation was found between the glyceollin precursor daidzein (4',7-dihydroxyisoflavone) and its conjugates and glyceollin levels in root tissues; however, isoflavone levels increased only in roots of inoculated plants of partially resistant lines, even though constitutive isoflavone levels were higher in the susceptible control. The FSG growth on potato dextrose agar medium was inhibited by increasing concentrations of glyceollin. Induction of lignin synthesis was found in the inoculated roots of all three lines, with the highest rate of lignification observed in roots of the partially resistant genotypes, especially PI 567374. These studies show for the first time that FSG inoculation of soybean roots in soil induces the phenylpropanoid pathway to synthesize isoflavones, the phytoalexin glyceollin, and lignin, indicating that these compounds may be involved in the partial resistance response.