Plant development regulated by cytokinin sinks
Plant development regulated by cytokinin sinks
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DOI：
10.1126/science.aaf7254
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发表时间：
2016-09-02
期刊：
影响因子：
56.9
通讯作者：
Muller, Bruno
中科院分区：
文献类型：
--
作者：
Zurcher, Evelyne;Liu, Jingchun;Muller, Bruno
形态发生信号控制多细胞生物的模式。细胞分裂素是由植物细胞亚群感知的移动的信号。我们发现拟南芥发育过程中对细胞分裂素信号的反应受到转运蛋白PURINE PERMEASE 14（PUP 14）的限制。在我们的实验中，PUP14的表达与细胞分裂素信号读出呈负相关。PUP14功能的丧失允许异位细胞分裂素信号伴随着胚胎、根和芽顶端分生组织的异常形态发生。PUP 14蛋白定位于质膜并输入生物活性细胞分裂素，从而耗尽质外体细胞分裂素库并抑制质膜定位的细胞分裂素传感器的感知以产生活性配体的汇。我们建议，时空细胞分裂素库模式建立的PUP14确定的细胞分裂素信号景观，形状的陆地植物的形态建成。
Morphogenetic signals control the patterning of multicellular organisms. Cytokinins are mobile signals that are perceived by subsets of plant cells. We found that the responses to cytokinin signaling during Arabidopsis development are constrained by the transporter PURINE PERMEASE 14 (PUP14). In our experiments, the expression of PUP14 was inversely correlated to the cytokinin signaling readout. Loss of PUP14 function allowed ectopic cytokinin signaling accompanied by aberrant morphogenesis in embryos, roots, and the shoot apical meristem. PUP14 protein localized to the plasma membrane and imported bioactive cytokinins, thus depleting apoplastic cytokinin pools and inhibiting perception by plasma membrane-localized cytokinin sensors to create a sink for active ligands. We propose that the spatiotemporal cytokinin sink patterns established by PUP14 determine the cytokinin signaling landscape that shapes the morphogenesis of land plants.