Quantitative assessment of the extent of myocardial infarction in the conscious dog by means of analysis of serial changes in serum creatine phosphokinase activity.
Quantitative assessment of the extent of myocardial infarction in the conscious dog by means of analysis of serial changes in serum creatine phosphokinase activity.
复制标题
通过分析血清肌酸磷酸激酶活性的系列变化来定量评估清醒狗的心肌梗塞程度。
DOI：
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发表时间：
1971
影响因子：
15.9
通讯作者：
B. Sobel
中科院分区：
文献类型：
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作者：
W. Shell;J. Kjekshus;B. Sobel
本研究旨在建立一种基于血清肌酸磷酸激酶（CPK）活性动态变化定量评估清醒动物脑梗死面积的方法。在清醒犬静脉注射心肌CPK的11次实验中，CPK在血清中的平均分布空间和平均消失率分别为体重的11.4%和0.48% min。在其他实验中，心肌梗死在22个清醒的狗左冠状动脉圈套器的收缩和血清CPK活性测定在频繁的时间间隔为24 hr. Since心肌CPK消耗反映了梗死面积，梗死面积直接确定通过分析心肌CPK含量在相同的动物冠状动脉闭塞后24小时。根据观察到的血清CPK活性的变化来确定从梗塞释放的CPK，所述血清CPK活性的变化根据考虑从梗塞释放的CPK的分数和从血清中CPK活性的出现和消失的速率的模型来分析。根据观察到的血清CPK变化计算梗死面积，并与通过分析心肌CPK耗竭直接确定的梗死面积进行比较。一致性接近，所有实验的结果符合方程：[根据血清CPK测定的梗死面积（g）] = 1.13 x [根据心肌CPK测定的梗死面积（g）] - 1.3，r = 0.96，n = 22。所描述的方法是有用的准确评估梗死面积在清醒的动物和检测修改的梗死面积产生的药理干预。
This study was designed to develop a method for quantitative assessment of infarct size in the conscious animal based on serial changes of serum creatine phosphokinase (CPK) activity. From 11 experiments in which myocardial CPK was injected intravenously in conscious dogs, the average CPK distribution space and average CPK fractional disappearance rate from serum were found to be 11.4% of body weight and 0.48% min respectively. In other experiments, myocardial infarction was produced in 22 conscious dogs by constriction of a left coronary artery snare and serum CPK activity was determined at frequent intervals for 24 hr. Since myocardial CPK depletion reflects infarct size, infarct size was determined directly by analysis of myocardial CPK content in the same animals 24 hr after coronary artery occlusion. CPK released from the infarct was determined from observed changes in serum CPK activity analyzed according to a model taking into account the fraction of CPK released from an infarct and the rates of appearance and disappearance of CPK activity from serum. Infarct size was calculated on the basis of observed changes in serum CPK and compared to infarct size determined directly by analysis of myocardial CPK depletion. Agreement was close and results from all experiments fit the equation: [infarct size (g) determined from serum CPK] = 1.13 x [infarct size (g) determined from myocardial CPK] - 1.3, r = 0.96, n = 22. The method described is useful for accurate assessment of infarct size in the conscious animal and for detection of modification of infarct size produced by pharmacologic interventions.