Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus.
Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus.
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DOI：
10.1038/srep27623
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发表时间：
2016-06-09
影响因子：
4.6
通讯作者：
Wang SX
中科院分区：
文献类型：
--
作者：
Lee JR;Haddon DJ;Wand HE;Price JV;Diep VK;Hall DA;Petri M;Baechler EC;Balboni IM;Utz PJ;Wang SX
高滴度、类别转换的自身抗体是系统性红斑狼疮（SLE）的标志。在活动性SLE患者中观察到干扰素（IFN）通路的失调，尽管特异性自身抗体与趋化因子评分（三种IFN调节的趋化因子的联合测量）的相关性尚不清楚。为了鉴定与趋化因子评分相关的自身抗体，我们开发了巨磁阻（GMR）生物传感器微阵列，其允许并行测量针对自身抗原和肽的多种血清抗体。我们使用微阵列分析SLE患者的血清样本，发现具有高趋化因子评分的个体对13种自身抗原的反应性显著高于具有低趋化因子评分的个体。我们的研究结果表明，多种自身抗体，包括抗体U1- 70 K和修改组蛋白H2 B尾巴，与干扰素失调SLE。此外，他们显示微阵列能够识别与SLE相关临床表现相关的自身抗体，具有在临床实践中用作生物标志物的潜力。
High titer, class-switched autoantibodies are a hallmark of systemic lupus erythematosus (SLE). Dysregulation of the interferon (IFN) pathway is observed in individuals with active SLE, although the association of specific autoantibodies with chemokine score, a combined measurement of three IFN-regulated chemokines, is not known. To identify autoantibodies associated with chemokine score, we developed giant magnetoresistive (GMR) biosensor microarrays, which allow the parallel measurement of multiple serum antibodies to autoantigens and peptides. We used the microarrays to analyze serum samples from SLE patients and found individuals with high chemokine scores had significantly greater reactivity to 13 autoantigens than individuals with low chemokine scores. Our findings demonstrate that multiple autoantibodies, including antibodies to U1-70K and modified histone H2B tails, are associated with IFN dysregulation in SLE. Further, they show the microarrays are capable of identifying autoantibodies associated with relevant clinical manifestations of SLE, with potential for use as biomarkers in clinical practice.