Antimicrobial Susceptibility, Tetracycline and Erythromycin Resistance Genes, and Multilocus Sequence Typing of Streptococcus suis Isolates from Diseased Pigs in China
Antimicrobial Susceptibility, Tetracycline and Erythromycin Resistance Genes, and Multilocus Sequence Typing of Streptococcus suis Isolates from Diseased Pigs in China
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DOI：
10.1292/jvms.12-0279
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发表时间：
2013-05-01
影响因子：
1.2
通讯作者：
Jin, Meilin
中科院分区：
文献类型：
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作者：
Chen, Lei;Song, Yajing;Jin, Meilin
猪链球菌（Streptococcus suis，S. suis）是一种新出现的人畜共患病原体，在养猪业中造成重大经济损失。本文研究了沙门氏菌对抗生素的敏感性、相关的耐药决定因素和序列类型。2008 - 2010年中国病猪分离株。在106株沙门氏菌中，血清2型是最常见的菌株（n=95）。收集的Sans菌株依次为血清型3（n=3）、5（n=3）、4（n=2）、7（n=1）、11（n=1）和28（n=1）。多位点序列分型结果显示，ST 1（n=21）和ST 7（n=74）是主要的ST，血清2型与ST 7（P=0.017，Fisher精确检验）和CC 1（P=0.024，Fisher精确检验）显著相关。药敏结果显示，对四环素的耐药率最高（99.1%），其次为阿奇霉素（68.9%）、红霉素（67.9%）、克林霉素（67.9%）、复方新诺明（16%）、左氧氟沙星（2.8%）、氯霉素（1.9%）、头孢克洛（0.9%）和头孢曲松（0.9%）。结果表明，在该菌株中可检测到泰特（M）、泰特（O）、泰特（O/W/32/O）、泰特（O/32/O）、泰特（S）、泰特（W）、泰特（L）、泰特（40）、tet 1（B）、mef（A/E）和msr（D）等抗生素耐药基因，并发现了多个抗生素耐药基因串联组织。结果表明，S.我国分离的猪源性大肠杆菌菌株多样性低，耐多药。
Streptococcus suis (S. suis) is an emerging zoonotic pathogen causing significant economic losses in the swine industry. Here, we investigated the antimicrobial susceptibility, associated antibiotic-resistant determinants and sequence type (ST) of S. suis isolates from diseased pigs in China from 2008 to 2010. Serotype 2 was the most frequently observed strain (n=95) among the 106 S. sans strains collected, followed by serotypes 3 (n=3), 5 (n=3), 4 (n=2), 7 (n=1), 11 (n=1) and 28 (n=1). Multilocus sequence typing analysis revealed that ST1 (n=21) and ST7 (n=74) were the predominant STs, and serotype 2 was found to be significantly correlated with ST7 (P=0.017, Fisher's exact test) and CC1 (P=0.024, Fisher's exact test). The antimicrobial susceptibility results indicated that the antibiotic resistance rate was highest for tetracycline (99.1%), followed by azithromycin (68.9%), erythromycin (67.9%), clindamycin (67.9%), trimethoprim/sulfamethoxazole (16%), levofloxacin (2.8%), chloramphenicol (1.9%), cefaclor (0.9%) and ceftriaxone (0.9%). Antibiotic-resistant genes tet(M), tet(O), tet(O/W/32/O), tet(O/32/O), tet(S), tet(W), tet(L), tet(40), erm(B), mef(A/E) and msr(D) could be detected, and several tandem organizations of antibiotic resistance genes were also found in this study. In conclusion, S. suis strains isolated from diseased pigs in China were less diverse and multi-drug resistant.