Genomic characteristics of a novel strain Lactiplantibacillus plantarum X7021 isolated from the brine of stinky tofu for the application in food fermentation
Genomic characteristics of a novel strain Lactiplantibacillus plantarum X7021 isolated from the brine of stinky tofu for the application in food fermentation
复制标题
从臭豆腐盐水中分离的新型植物乳杆菌X7021的基因组特征及其在食品发酵中的应用
DOI：
10.1016/j.lwt.2021.113054
复制
发表时间：
2022-01-08
影响因子：
6
通讯作者：
Wei, Dongzhi
中科院分区：
文献类型：
--
作者：
Liu, Guang;Liu, Yafan;Wei, Dongzhi
植物乳杆菌X7021是从臭豆腐盐水中分离得到的一株新菌株。为了探讨该菌株在食品工业中的应用潜力，利用比较基因组学和转录检测技术对该菌株的基因组特征和部分代谢特征进行了研究。X7021的基因组全长为3,407,054个碱基，含3259个蛋白质编码序列和6个质粒。选择植物乳杆菌菌株WCFS1、JDM1和ST-III进行比较基因组分析。结果表明，X7021在抗生素耐药性和毒力基因较少的食品中应用是安全的。该菌株具有25个完整的磷酸转移酶系统转运体和较强的蛋白分解系统，对多种食物具有适应性。该菌株含有完整的嘌呤代谢途径，在豆奶发酵过程中鸟嘌呤、次黄嘌呤和黄嘌呤的降解率分别为38.9%、26.1%和70.2%。大豆异黄酮苷可以被两种β-葡萄糖苷酶转化为相应的苷元。利用高效的亚硝酸盐转运蛋白和亚硝酸还原酶，该菌株能在700 mg/L亚硝酸盐中生长，并在24 h内完全降解100 mg/L亚硝酸盐。因此，植物乳杆菌X7021是一种很有前景的植物食品发酵发酵剂。
Lactiplantibacillus plantarum X7021 is a novel strain originated from the brine of stinky tofu. In order to investigate the application potential of the strain in food industry, the genomic characteristics as well as some metabolic features were studied using comparison genomics and transcriptional assay. The complete genome of X7021includes a 3,407,054 bp chromosome with 3259 protein coding sequences, and 6 plasmids. L. plantarum strains, WCFS1, JDM1, and ST-III were selected for comparative genomic analysis. Results revealed that X7021 is safe for the application in foods with less antibiotic resistance and virulence genes. The strain has 25 complete phosphotransferase system transporters and strong proteolytic system for its adaptability to various foodstuffs. The strain containes a complete purine metabolic pathway incorporating 4 permeases, resulting in the reductions of 38.9% guanine, 26.1% hypoxanthine and 70.2% xanthine during the soymilk fermentation. Soy isoflavone glycosides could be converted to corresponding aglycones by two beta-glucosidases. The strain could grow in 700 mg/L nitrite and entirely degrade 100 mg/L nitrite during 24 h based on highly active nitrite transporter and nitrite reductase. Accordingly, the results suggest that L. plantarum X7021 is a prospect starter candidate for the fermentation of plant foodstuffs.