NOVEL USE OF POLYMERASE CHAIN-REACTION TO AMPLIFY CELLULAR DNA ADJACENT TO AN INTEGRATED PROVIRUS
NOVEL USE OF POLYMERASE CHAIN-REACTION TO AMPLIFY CELLULAR DNA ADJACENT TO AN INTEGRATED PROVIRUS
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DOI：
10.1128/jvi.63.5.1924-1928.1989
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发表时间：
1989-05-01
影响因子：
5.4
通讯作者：
KEERIKATTE, V
中科院分区：
文献类型：
--
作者：
SILVER, J;KEERIKATTE, V
我们描述了一种改进的聚合酶链式反应技术，它允许在给定仅针对前病毒的序列信息的情况下，扩增与整合的前病毒相邻的细胞DNA。这种改进的技术对于插入突变的研究和其他希望分离与已知序列区域相邻的DNA的情况通常是有用的。
We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which one wishes to isolate DNA adjacent to a region of known sequence.