Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration
Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration
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DOI：
10.1002/pmic.200701091
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发表时间：
2008-10-01
期刊：
影响因子：
3.4
通讯作者：
Osta, Rosario
中科院分区：
文献类型：
--
作者：
Calvo, Ana C.;Moreno-Igoa, Maria;Osta, Rosario
内标的看家蛋白或基因的选择应仔细考虑细胞和组织类型、实验条件和正在考虑的健康/疾病状态(S)。此外，由于常用看家基因的转录和翻译水平之间的相关性经常被讨论，本研究揭示了肌萎缩侧索硬化症小鼠模型中P-肌动蛋白和3-磷酸甘油醛脱氢酶(GAPDH)的转录水平以及β-肌动蛋白、GAPDH和β-微管蛋白的翻译水平。
The choice of housekeeping proteins or genes for internal standards should be made carefully, taking into account the cell and tissue type, the experimental conditions, and the healthy/disease state(s) under consideration. Furthermore, as the correlation between transcriptional and translational levels of commonly used housekeeping genes is often discussed, this study shed light on the transcriptional levels of P-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the translational levels of beta-actin, GAPDH, and beta-tubulin in an amyotrophic lateral sclerosis mouse model.