Paramagnetic 19F NMR and Electrospray Ionization Mass Spectrometric Studies of Substituted Pyridine Complexes of Chromium(III): Models for Potential Use of 19F NMR to Probe Cr(III)-Nucleotide Interaction.
Paramagnetic 19F NMR and Electrospray Ionization Mass Spectrometric Studies of Substituted Pyridine Complexes of Chromium(III): Models for Potential Use of 19F NMR to Probe Cr(III)-Nucleotide Interaction.
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铬 (III) 取代吡啶配合物的顺磁 19F NMR 和电喷雾电离质谱研究：潜在使用 19F NMR 探测 Cr(III)-核苷酸相互作用的模型。
DOI：
10.1016/j.poly.2013.03.002
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发表时间：
2013
期刊：
影响因子：
2.6
通讯作者：
Vincent,JohnB
中科院分区：
文献类型：
--
作者：
Rhodes,NicholasR;Belmore,Ken;Cassady,CarolynJ;Vincent,JohnB
报道了含三氟乙酸酯、3-氟吡啶、3-三氟甲基吡啶和4-三氟甲基吡啶的铬碱甲酸络合物[Cr3(O2CR)6L3]+的合成和表征。取代的吡啶配体被用来作为DNA碱基的模型，以确定19F核磁共振是否可以作为潜在的有用的探针来研究Cr3+与DNA的结合。配位配体的~(19)F核磁共振谱，虽然通过未配对电子密度的离域而从3/2个色度中心被展宽，但很容易辨别，并且接触位移足够大，可以很容易地区分来自配位配体和自由配体的信号。因此，19F核磁共振似乎是一种潜在的有用的探针，研究Cr3+与含有F标记碱基的DNA的结合。此外，电喷雾质谱被证明是确定碱性羧酸铬组装体的一种方便的方法。
The synthesis and characterization of chromium basic carboxylate complexes, [Cr3(O2CR)6L3]+, containing trifluoroacetate, 3-fluoropyridine, 3-trifluoromethylpyridine, and 4-trifluoromethylpyridine are described. The substituted pyridine ligands are used as models of DNA bases to determine whether19F NMR would be a potentially useful probe of the binding of Cr3+to DNA. The19F NMR resonances of the coordinated ligands, while broadened by delocalization of unpaired electron density from theS= 3/2 chromic centers, are readily discernable, and the contact shifts are of sufficient magnitude that the signals from coordinated and free ligands can easily be differentiated. Thus,19F NMR appears to be a potentially useful probe of the binding of Cr3+to DNA containing F-labeled bases. Additionally, electrospray MS is shown to be a convenient method to establish the identity of chromium basic carboxylate assemblies.