Identification of lignans by liquid chromatography-electrospray ionization ion-trap mass spectrometry
Identification of lignans by liquid chromatography-electrospray ionization ion-trap mass spectrometry
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DOI：
10.1002/jms.1276
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发表时间：
2008-01-01
影响因子：
2.3
通讯作者：
Sjoholm, Rainer E.
中科院分区：
文献类型：
--
作者：
Eklund, Patrik C.;Backman, M. Josefin;Sjoholm, Rainer E.
采用液相色谱-电喷雾电离离子阱质谱法研究了属于木脂素家族不同类别的 30 种化合物的裂解模式。根据观察到的碎片模式，实现了不同类型木脂素的鉴定。例如，二苄基丁内酯木脂素通过内酯部分损失 44 Da (CO2) 显示出特征性断裂途径，而二苄基丁二醇通过 1,4-丁二醇部分的甲醛和水的组合损失显示出 48 Da 的损失。木脂素糖苷很容易失去糖残基，得到母体木脂素作为其主要产物离子。此外，还观察到了几种化合物特异性的碎片，并用于鉴定单个化合物。开发了一种分析木酚素的通用方法，该方法使用 C8 柱上的 LC 分离，然后碎片化并检测离子阱中产生的离子。版权所有 (c) 2007 John Wiley & Sons, Ltd.
The fragmentation pattern of 30 compounds belonging to different classes of the lignan family was studied by liquid chromatography-electrospray ionization ion-trap mass spectrometry. On the basis of the observed fragmentation patterns, identification of different types of lignans was achieved. For example, dibenzylbutyrolactone lignans showed a characteristic fragmentation pathway by the loss of 44 Da (CO2) from the lactone moiety, whereas dibenzylbutanediols showed a loss of 48 Da by a combined loss of formaldehyde and water from the 1,4-butanediol moiety. Lignan glycosides readily lost the sugar residue to give the parent lignan as their primary product ion. In addition, several compound-specific fragmentations were observed and used for identification of individual compounds.A versatile method for analyses of lignans was developed using LC separation on a C8 column followed by fragmentation and detection of ions produced in the ion trap. Copyright (c) 2007 John Wiley & Sons, Ltd.