Primitive templated catalysis of a peptide ligation by self-folding RNAs.
Primitive templated catalysis of a peptide ligation by self-folding RNAs.
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DOI：
10.1093/nar/gkp111
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发表时间：
2009-05
影响因子：
14.9
通讯作者：
Ikawa Y
中科院分区：
文献类型：
--
作者：
Kashiwagi N;Furuta H;Ikawa Y
rna -多肽复合物（RNA-polypeptide complexes, RNPs）在现存生物系统中发挥着多种作用，在生命分子进化的早期阶段起着重要作用。在古代RNPs的一定发育阶段，它们的RNA和多肽成分被认为是相互进化的，以建立高度复杂的结构和功能。我们已经构建了一个简单的模型系统，以此为基础可以发展RNA和多肽组分的协同进化系统。基于对几种rna适度加速两种基本肽的化学连接的观察。我们设计了一种具有两个肽结合位点的RNA分子来捕获这两个肽。所设计的RNA还可以加速肽的连接。由此产生的连接肽具有两个RNA结合位点，可以反过来作为反式作用因子，增强所设计的RNA催化的内切酶活性。
RNA–polypeptide complexes (RNPs), which play various roles in extant biological systems, have been suggested to have been important in the early stages of the molecular evolution of life. At a certain developmental stage of ancient RNPs, their RNA and polypeptide components have been proposed to evolve in a reciprocal manner to establish highly elaborate structures and functions. We have constructed a simple model system, from which a cooperative evolution system of RNA and polypeptide components could be developed. Based on the observation that several RNAs modestly accelerated the chemical ligation of the two basic peptides. We have designed an RNA molecule possessing two peptide binding sites that capture the two peptides. This designed RNA can also accelerate the peptide ligation. The resulting ligated peptide, which has two RNA-binding sites, can in turn function as a trans-acting factor that enhances the endonuclease activity catalyzed by the designed RNA.
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