Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix
Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix
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DOI：
10.1002/jbm.b.32897
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发表时间：
2013-08-01
影响因子：
3.4
通讯作者：
Kim, Wan Hee
中科院分区：
文献类型：
--
作者：
Choi, Kyuseok;Kang, Byung-Jae;Kim, Wan Hee
本研究探讨了脂肪源性间充质干细胞（ASC）种子脱细胞真皮基质（ADM）联合低水平激光治疗（LLLT）修复胸腺裸鼠骨缺损的可行性。用ADM、ADM/LLLT、ADM/ASCs或ADM/ASCs/LLLT治疗临界尺寸的颅骨缺损。在显微计算机断层扫描中，ADM/ASCs组和ADM/ASCs/LLLT组在14天后显示出显著的骨形成。此外，ADM/ASCs/LLLT组的骨再生在28天明显，而ADM/ASCs组在56天。ADM/ASCs/LLLT组骨矿物质密度和骨组织体积在7 d后显著升高，而ADM/ASCs组在14 d后显著升高。组织学分析显示，ADM/ASCs组和ADM/ASCs/LLLT组缺损均得到修复，而ADM和ADM/LLLT组缺损在28和56 d时出现少量骨岛。证实了ASCs在ADM上的成功播种，并且LLLT在14 d时增强了ASCs的增殖和存活。我们的研究结果表明，asc种子移植物促进骨再生，并且在asc种子ADM上应用LLLT导致骨快速形成。asc种子ADM与LLLT联合植入可有效地用于骨再生。(c) 2013 Wiley Periodicals, Inc.；[J]中国生物医学工程学报，2013。
This study investigates the feasibility of using an adipose-derived mesenchymal stem cell (ASC)-seeded acellular dermal matrix (ADM) along with low-level laser therapy (LLLT) to repair bone defect in athymic nude mice. Critical-sized calvarial defects were treated either with ADM, ADM/LLLT, ADM/ASCs, or ADM/ASCs/LLLT. In micro-computed tomography scans, the ADM/ASCs and the ADM/ASCs/LLLT groups showed remarkable bone formation after 14 days. Additionally, bone regeneration in the ADM/ASCs/LLLT group was obvious at 28 days, but in the ADM/ASCs group at 56 days. Bone mineral density and bone tissue volume in the ADM/ASCs/LLLT group significantly increased after 7 days, but in the ADM/ASCs group after 14 days. Histological analysis revealed that the defects were repaired in the ADM/ASCs and the ADM/ASCs/LLLT group, while the defects in the ADM and the ADM/LLLT groups exhibited few bone islands at 28 and 56 days. The successful seeding of ASCs onto ADM was confirmed, and LLLT enhanced the proliferation and the survival of ASCs at 14 days. Our results indicate that ASC-seeded grafts promote bone regeneration, and the application of LLLT on ASC-seeded ADM results in rapid bone formation. The implantation of an ASC-seeded ADM combined with LLLT may be used effectively for bone regeneration. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.