CRISPR Guide RNA Design Guidelines for Efficient Genome Editing
CRISPR Guide RNA Design Guidelines for Efficient Genome Editing
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DOI：
10.1007/978-1-0716-0712-1_19
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发表时间：
2020-01-01
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影响因子：
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通讯作者：
Puchta, Holger
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文献类型：
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作者：
Schindele, Patrick;Wolter, Felix;Puchta, Holger
CRISPR/ cas9系统的简单适用性和容易的靶标编程消除了以往基因组编辑工具的主要局限性，使其成为产生位点特异性基因组改变的首选工具。它的多功能性和功效已在各种生物体中得到证实；然而，准确预测向导RNA的效率仍然是一个与生物体无关的挑战。因此，设计最佳的引导rna对于最大化实验结果至关重要。在这里，我们总结了当前的知识，引导RNA设计和突出不同的实验系统之间的差异。
The simple applicability and facile target programming of the CRISPR/Cas9-system abolish the major boundaries of previous genome editing tools, making it the tool of choice for generating site-specific genome alterations. Its versatility and efficacy have been demonstrated in various organisms; however, accurately predicting guide RNA efficiencies remains an organism-independent challenge. Thus, designing optimal guide RNAs is essential to maximize the experimental outcome. Here, we summarize the current knowledge for guide RNA design and highlight discrepancies between different experimental systems.