Fundamental aspects of protein-protein association kinetics.
Fundamental aspects of protein-protein association kinetics.
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DOI：
10.1021/cr800373w
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发表时间：
2009-03-11
期刊：
影响因子：
62.1
通讯作者：
Zhou, H-X
中科院分区：
文献类型：
--
作者：
Schreiber, G.;Haran, G.;Zhou, H-X
蛋白质复合物的结构连同关于其亲和力和其他热力学特征的信息提供了复合物的“冻结”视图。这幅图忽略了蛋白质-蛋白质缔合和解离的动力学性质，这是主要的生物学和生物物理学兴趣。本文综述了蛋白质复合物形成动力学途径的最新研究进展，（我们称之为“瞬态复合物”1），过渡态，和其他中间体蛋白质-蛋白质结合是多种生物过程的中心，从酶催化到蛋白质结合，抑制细胞因子对免疫应答的调节。在这种过程中，关联率通常起着关键作用，例如在速度至关重要的情况下。[2]例如，紫锥蜗牛和其他有毒动物通过释放毒素迅速与离子通道结合，以惊人的效率和速度捕获猎物;[3]绿色曼巴蛇通过靶向乙酰胆碱酯酶（AChE）实现了类似的壮举，AChE是一种对神经传递的完整性至关重要的酶。4
The structure of a protein complex together with information about its affinity and other thermodynamic characteristics provide a “frozen” view of the complex. This picture ignores the kinetic nature of protein-protein association and dissociation, which is of major biological and biophysical interest. This review focuses on recent advances in deciphering the kinetic pathway of protein complex formation, the nature of the precomplex formed through diffusion (which we termed the “transient complex” 1), the transition state, and other intermediates (such as the so-called encounter complex) along the association pathway.Protein-protein association is at the center of diverse biological processes ranging from enzyme catalysis/inhibition to regulation of immune response by cytokines. The association rates often play a critical role in such processes, as in situations where speed is of essence. 2 For example, the purple cone snail and other venomous animals capture prey with remarkable efficiency and speed by releasing toxins that rapidly bind to ion channels; 3 the green mamba achieves a similar feat by targeting acetylcholinesterase (AChE), an enzyme essential for the integrity of neural transmission. 4
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