Proliferative growth of SARS coronavirus in Vero E6 cells
Proliferative growth of SARS coronavirus in Vero E6 cells
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DOI：
10.1099/vir.0.19505-0
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发表时间：
2003-12-01
影响因子：
3.8
通讯作者：
Ling, AE
中科院分区：
文献类型：
--
作者：
Ng, ML;Tan, SH;Ling, AE
从患者体内获得 SARS 冠状病毒分离株（毒株 2003VA2774），用于感染 Vero E6 细胞。感染后 1 至 30 小时 (p.i.) 跟踪病毒的复制周期。观察到这种人类病毒在 Vero 细胞中的快速生长令人惊讶。在感染的第一个小时内，最明显的超微结构变化是高尔基复合体和相关囊泡的增殖，并伴有一些跨高尔基囊的肿胀。注射后 5 小时出现细胞外病毒颗粒。大约 5% 的细胞中，这一比例在一小时内（注射后 6 小时）急剧增加到细胞群的 30% 左右。肿胀的高尔基囊含有处于不同成熟阶段的病毒核衣壳。这些病毒前体也存在于大液泡中，并与膜轮紧密相连。膜轮可能是复制复合体，因为它们出现在复制周期的早期。随着感染从感染后 12 小时发展到 21 小时，受感染细胞的细胞质充满了许多大的、膜光滑的液泡，其中含有成熟病毒和球形核心的混合物。其中几个液泡靠近细胞外围，准备通过胞吐作用输出成熟的子代病毒颗粒。注射后 24 至 30 小时，细胞表面常见胞外病毒颗粒的晶体阵列。
An isolate of SARS coronavirus (strain 2003VA2774) was obtained from a patient and used to infect Vero E6 cells. The replication cycle of the virus was followed from 1 to 30 h post-infection (p.i.). It was surprising to observe the swift growth of this human virus in Vero cells. Within the first hour of infection, the most obvious ultrastructural change was the proliferation of the Golgi complexes and related vesicles accompanied by swelling of some of the trans-Golgi sacs. Extracellular virus particles were present by 5 h p.i. in about 5% of the cells and this increased dramatically to about 30% of the cell population within an hour (6 h p.i.). Swollen Golgi sacs contained virus nucleocapsids at different stages of maturation. These virus precursors were also in large vacuoles and in close association with membrane whorls. The membrane whorls could be the replication complexes, since they appeared rather early in the replication cycle. As infection progressed from 12 to 21 h p.i., the cytoplasm of the infected cells was filled with numerous large, smooth-membraned vacuoles containing a mixture of mature virus and spherical cores. Several of these vacuoles were close to the cell periphery, ready to export out the mature progeny virus particles via exocytosis. By 24 to 30 h p.i., crystalline arrays of the extracellular virus particles were seen commonly at the cell surface.