Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms
Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms
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DOI：
10.1038/nmeth.1355
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发表时间：
2009-09-01
期刊：
影响因子：
48
通讯作者：
Schwille, Petra
中科院分区：
文献类型：
--
作者：
Ries, Jonas;Yu, Shuizi Rachel;Schwille, Petra
体内受体-配体相互作用的分析是生物学的关键，但对定量显微镜构成了相当大的挑战。在这里，我们结合联合收割机静态体积，双焦点和双色扫描荧光相关光谱，以解决这个问题，在复杂的生物环境中的细胞分辨率。我们定量的成纤维细胞生长因子受体Fgfr 1和Fgfr 4在活斑马鱼胚胎的细胞膜的流动性，并确定其在体内的结合亲和力，其配体Fgf 8。(C)2009年自然美国公司All rights reserved.
Analysis of receptor-ligand interactions in vivo is key to biology but poses a considerable challenge to quantitative microscopy. Here we combine static-volume, two-focus and dual-color scanning fluorescence correlation spectroscopy to solve this task at cellular resolution in complex biological environments. We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8. (C) 2009 Nature America, Inc. All rights reserved.