Effect of affinity for droplet surfaces on the fraction of analyte molecules charged during electrospray droplet fission
Effect of affinity for droplet surfaces on the fraction of analyte molecules charged during electrospray droplet fission
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DOI：
10.1021/ac001267j
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发表时间：
2001-10-01
影响因子：
7.4
通讯作者：
Enke, CG
中科院分区：
文献类型：
--
作者：
Cech, NB;Enke, CG
探讨了电喷雾过程中液滴质量和电荷的不均匀分裂对分析物带电量的影响。一个表面选择性因子（S）被开发来描述的液滴表面的分析物的亲和力，和理论和实验的响应曲线进行了比较与不同的S值的分析物。通过计算电荷和从母液滴产生的分析物之间的重叠来产生理论响应曲线，以确定带电的分析物的量。然后将该重叠作为分析物浓度的函数作图。液滴分裂过程中带电的分析物的量的差异预测不同的表面亲和力的分析物。分析物在ESI液滴的表面相和内部相之间的分配问题也包括在讨论中。这是通过将平衡分配模型应用于一组后代液滴以确定其表面上的分析物的量，然后计算裂变分析物和过量电荷之间的重叠来实现的。实验响应曲线与理论响应曲线相似，从理论预测的S值与根据分析物的结构特征预测的S值非常一致。
The effect of uneven fissioning of mass and charge from electrospray droplets on the amount of analyte charged during the electrospray process was explored. A surface selectivity factor (S) was developed to describe the affinity of an analyte for the droplet surface, and both theoretical and experimental response curves were compared for analytes with various S values. The theoretical response curves were generated by calculating the overlap between the charge and analyte spawned from parent droplets to determine the amount of analyte charged. This overlap was then graphed as a function of analyte concentration. Differences in the amount of analyte charged during droplet fission were predicted for analytes of varying surface affinities. The issue of analyte partitioning between the surface and interior phases of the ESI droplet was also included in the discussion. This was accomplished by applying the equilibrium partitioning model to a set of offspring droplets to determine the amount of analyte on their surfaces and then calculating the overlap between fissioning analyte and excess charge. Experimental response curves resembled theoretical ones, and S values predicted from theory were in excellent agreement with those predicted on the basis of the structural characteristics of the analytes.