High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide production.
High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide production.
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恶臭假单胞菌 HKT554 菌株的高细胞密度培养及其在光学活性亚砜生产中的应用
DOI：
10.1007/s00253-012-4445-x
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发表时间：
2013
影响因子：
5
通讯作者：
Hirofumi Minami
中科院分区：
文献类型：
--
作者：
Said Hamad Ramadhan;Toru Matsui;Kazuma Nakano;Hirofumi Minami
对恶臭假单胞菌（Pseudomonasputida）HKT 554菌株的培养条件进行了优化，该菌株表达萘双加氧酶，具有广泛的底物特异性。在改良的TK-B培养基中，采用葡萄糖分批补料和溶氧状态，培养40小时，菌体密度达114 g干菌体/l。将该体系进一步应用于由甲基苯硫醚合成S-（+）-甲基苯基亚砜（MPSO）。添加非极性有机溶剂，如正十六烷，大大提高了MPSO的生产，由于防止底物蒸发，导致MPSO生产高达39 mM，在30 h内的转化率为95.7 mol%。
Culture conditions withPseudomonas putidastrain HKT554, expressing naphthalene dioxygenase, known as the biocatalyst showing wide substrate specificity, were optimized for high cell density cultivation (HCDC). Culture in a medium TK-B modified from that for HCDC ofEscherichia coliwith glucose fed-batch and dissolved oxygen stat resulted in a high cell density growth of 114 g dry cell/l at 40 h of cultivation. This system was further applied for S-(+)-methyl phenyl sulfoxide (MPSO) production from methyl phenyl sulfide. Addition of nonpolar organic solvent, such asn-hexadecane, greatly enhanced the MPSO production due to the prevention of substrate evaporation, resulting in a MPSO production up to 39 mM in 30 h with a conversion rate of 95.7 mol%.