Transcriptional Reprogramming of Autographa Californica Multiple Nucleopolyhedrovirus Chitinase and Cathepsin Genes Enhances Virulence.
Transcriptional Reprogramming of Autographa Californica Multiple Nucleopolyhedrovirus Chitinase and Cathepsin Genes Enhances Virulence.
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DOI：
10.3390/v15020503
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发表时间：
2023-02-11
期刊：
影响因子：
--
通讯作者：
Krell PJ
中科院分区：
文献类型：
--
作者：
Hodgson JJ;Passarelli AL;Krell PJ
杆状病毒几丁质酶（CHIA）和组织蛋白酶（V-CATH）酶促进终末昆虫宿主液化，这有助于病毒子代传播。重组苜蓿银纹夜蛾核型多角体病毒（AcMNPV）衍生的病毒先前通过用AcMNPV多角体蛋白（polh）或核心蛋白（p6.9）启动子序列替换天然启动子而用重编程的chiA转录产生，但这两种chiA重编程的病毒都缺乏v-cath转录和V-CATH酶活性。在这里，我们报告说，双p6.9/polh启动子重编程的相邻的chiA/v-cath基因导致调制的时间转录的两个基因，而不影响感染性出芽病毒的生产。这些启动子的变化增加了CHIA和V-CATH酶活性在感染的草地贪夜蛾衍生的培养细胞和粉纹夜蛾幼虫。此外，感染双重重编程病毒的幼虫有较早的死亡和液化。这种重组杆状病毒缺乏外源基因组元件和增加的chiA/v-cath表达水平，可能是生产增强的基于杆状病毒的生物农药所需的并且适合于生产增强的基于杆状病毒的生物农药。
The baculoviral chitinase (CHIA) and cathepsin (V-CATH) enzymes promote terminal insect host liquefaction, which aids viral progeny dissemination. Recombinant Autographa californica nucleopolyhedrovirus (AcMNPV)-derived viruses were previously generated with reprogrammed chiA transcription by replacing the native promoter with the AcMNPV polyhedrin (polh) or core protein (p6.9) promoter sequences, but of both these chiA-reprogrammed viruses lacked v-cath transcription and V-CATH enzymatic activity. Here, we report that dual p6.9/polh promoter reprogramming of the adjacent chiA/v-cath genes resulted in modulated temporal transcription of both genes without impacting infectious budded virus production. These promoter changes increased CHIA and V-CATH enzyme activities in infected Spodoptera frugiperda-derived cultured cells and Trichoplusia ni larvae. In addition, larvae infected with the dual reprogrammed virus had earlier mortalities and liquefaction. This recombinant baculovirus, lacking exogenous genomic elements and increased chiA/v-cath expression levels, may be desirable for and amenable to producing enhanced baculovirus-based biopesticides.
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影响因子：
3.8
作者：
Hodgson, Jeffrey J.;Arif, Basil M.;Krell, Peter J.
通讯作者：
Krell, Peter J.
影响因子：
5.6
作者：
OOI, BG;RANKIN, C;MILLER, LK
通讯作者：
MILLER, LK
影响因子：
3.8
作者：
GOPALAKRISHNAN, B;MUTHUKRISHNAN, S;KRAMER, KJ
通讯作者：
KRAMER, KJ
影响因子：
5.4
作者：
Hodgson, Jeffrey J.;Arif, Basil M.;Krell, Peter J.
通讯作者：
Krell, Peter J.
影响因子：
2.7
作者：
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通讯作者：
Shimada, T.