APPLICATIONS OF NATURAL-ABUNDANCE NITROGEN-15 NUCLEAR MAGNETIC-RESONANCE TO LARGE BIOCHEMICALLY IMPORTANT MOLECULES
APPLICATIONS OF NATURAL-ABUNDANCE NITROGEN-15 NUCLEAR MAGNETIC-RESONANCE TO LARGE BIOCHEMICALLY IMPORTANT MOLECULES
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DOI：
10.1073/pnas.72.12.4696
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
ROBERTS, JD
中科院分区：
文献类型：
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作者：
GUST, D;MOON, RB;ROBERTS, JD
利用最新可用的仪器，酶和其他生物聚合物的自然丰度氮-15核磁共振波谱被发现是可行的。这种分子的长关联时间导致短的自旋-晶格弛豫时间，而这反过来又允许信号的快速积累。然而，短T1值的优点有时会被不利的核Overhaser效应所抵消。讨论了T1和核Overhauser效应与关联时间的关系，并给出了几种生物聚合物，包括溶菌酶、鱼精蛋白、胃酶、血红蛋白、维生素B12和tRNA的初步氮-15核磁共振结果。
Natural-abundance nitrogen-15 nuclear magnetic resonance spectroscopy of enzymes and other biopolymers is found to be feasible using newly available instrumentation. The long correlation times of such molecules result in short spin-lattice relaxation times, and these in turn allow rapid signal accumulation. The advantages of short T1 values are sometimes offset, however, by unfavorable nuclear Overhauser effects. The dependence of T1 and nuclear Overhauser effects upon correlation time is discussed, and preliminary nitrogen-15 nuclear magnetic resonance results for several biopolymers, including lysozyme, protamines, pepsin, hemoglobin, vitamin B 12, and tRNA, are presented.