Effects of butyl paraben on the male reproductive system in mice
Effects of butyl paraben on the male reproductive system in mice
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DOI：
10.1007/s00204-002-0360-8
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发表时间：
2002-07-01
影响因子：
6.1
通讯作者：
Oishi, S
中科院分区：
文献类型：
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作者：
Oishi, S
对羟基苯甲酸酯是对羟基苯甲酸的烷基酯化合物，广泛用于食品、化妆品、化妆品和药品中的防腐剂。已知这些化合物在体外雌激素受体检测和体内子宫营养检测中具有较弱的雌激素样活性。在本文中，我们证明了对羟基苯甲酸丁酯对雄性小鼠生殖系统的不利影响，并损害了睾丸中精子发生的后期步骤。4周龄CRJ：CD-1小鼠随机分为8组，分别以0.01%、0.10%和1.00%的剂量饲喂帕拉苯丁酯10周。在0.01%、0.10%和1.00%的饮食中，平均每日摄入量分别为14.4+/-3.60、146+/-35.9和1504+/-357 mg/kg。在任何研究组中，对羟基苯甲酸丁酯对肝脏、腹侧前列腺、精囊和包皮腺(无论是绝对重量还是相对体重)都没有治疗相关的影响。1.00%组附睾体的绝对重量和相对重量均显著高于对照组。在VII-VIII生精小管中观察到圆形和细长精子细胞计数均呈剂量依赖性减少，且所有处理组的细长精子细胞计数均显著低于对照组。精原细胞和精母细胞的数量与对照无明显差异。血清睾酮浓度呈剂量依赖性下降，以1.00%为显著。这些数据表明，在远低于日本每日可接受摄入量(ADI)的剂量下，对羟基苯甲酸丁酯会对男性生殖系统产生不利影响。
Parabens are alkyl ester compounds of p-hydroxybenzoic acid widely used as preservatives in foodstuffs, cosmetics, toiletries and pharmaceuticals. These compounds are known to exert a weak estrogenic activity in estrogen receptor assays in vitro and in uterotrophic assays in vivo. In this paper, we have shown that butyl paraben had an adverse effect on the male mouse reproductive system and that it damaged the late steps of spermatogenesis in the testis. Butyl paraben was administered to 4-week-old Crj:CD-1 mice assigned to groups of eight animals, at doses of 0.01%, 0.10%, and 1.00% in the diet for 10 weeks. The average butyl paraben intake from the calculated food consumption was 14.4+/-3.60, 146+/-35.9, and 1504+/-357 mg/kg per day for the 0.01%, 0.10%, and 1.00% dietary butyl paraben groups, respectively. There were no treatment-related effects of butyl paraben on the liver, ventral prostates, seminal vesicles, and preputial glands (both in terms of absolute weight and relative to body weight) in any of the study groups. Both the absolute and relative weights of the epididymides were significantly higher in 1.00% group when compared with controls. A dose-dependent decrease of both round and elongated spermatid counts in stages VII-VIII seminiferous tubules was observed, and the elongated spermatid counts were significantly lower in all of the treated groups. The numbers of spermatogonia and spermatocytes did not differ from control values. The serum testosterone concentration decreased in a dose-dependent fashion and was significant at 1.00%. These data demonstrated that butyl paraben can exert an adverse effect on the male reproductive system at doses that are well below those of the accepted daily intake (ADI) in Japan.