DIAGNOSIS OF CREUTZFELDT-JAKOB DISEASE BY WESTERN-BLOT IDENTIFICATION OF MARKER PROTEIN IN HUMAN-BRAIN TISSUE
DIAGNOSIS OF CREUTZFELDT-JAKOB DISEASE BY WESTERN-BLOT IDENTIFICATION OF MARKER PROTEIN IN HUMAN-BRAIN TISSUE
复制标题
DOI：
10.1056/nejm198602273140904
复制
发表时间：
1986-02-27
影响因子：
158.5
通讯作者：
GAJDUSEK, DC
中科院分区：
文献类型：
--
作者：
BROWN, P;COKERVANN, M;GAJDUSEK, DC
我们检测了39例克雅氏病、Gerstmann-Straussler-Scheinker综合征或库鲁病患者和32例各种非海绵状变性疾病患者的脑组织纯化制剂，并使用抗血清进行Western印迹，该抗血清来自羊瘙痒病感染的仓鼠脑。31例克雅氏病患者的标本中，81%呈阳性反应（以及冷冻储存不到一年的所有7份标本），库鲁病患者的4份标本中的3份，Gerstmann-Straussler-Scheinker综合征患者的4份标本中的3份，其他神经系统退行性疾病患者的样本中无一例，包括家族性或散发性阿尔茨海默病;与肌阵挛、运动神经元病或帕金森综合征相关的痴呆;获得性免疫缺陷综合征脑病。因此，免疫学检测已开始为海绵状脑病的诊断提供神经病理学检查和动物传播实验的有用和快速的辅助手段。
We tested purified preparations of brain tissue from 39 patietns with Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker syndrome, or kuru, and from 32 patietns with a variety of nonspongiform degenerative diseases, with the use of Western blots against an antiserum to a similarly purified fraction made from scrapie-infected hamster brain. Positive reactions occurred in 81 percent of the 31 specimens from the patients with Creutzfeldt-Jakob disease (and in all of the 7 specimens that were stored frozen for less than one year), in 3 of the 4 specimens from the patients with kuru, in 3 of the 4 specimens from the patients with Gerstmann-Straussler-Scheinker syndrome, and in none of the specimens from the patients with other neurologic degenerative disorders, including familial or sporadic Alzheimer''s disease; dementia associated with myoclonus, motor neuron disease, or parkinsonism; and acquired-immunodeficiency-syndrome encephalopathy. Immunologic testing has thus begun to provide a useful and rapid adjunct to neuropathological examinations and animal-transmission experiments for the diagnosis of the spongiform encephalopathies.