Clonotypic analysis of cerebrospinal fluid T cells during disease exacerbation and remission in a patient with multiple sclerosis
Clonotypic analysis of cerebrospinal fluid T cells during disease exacerbation and remission in a patient with multiple sclerosis
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DOI：
10.1016/j.jneuroim.2005.10.002
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Martin, R
中科院分区：
文献类型：
--
作者：
Muraro, PA;Cassiani-Ingoni, R;Martin, R
自身反应性T细胞向中枢神经系统（CNS）的迁移被认为是多发性硬化（MS）发病机制中的重要步骤。为了跟踪复发缓解型MS患者CNS中T细胞库的演变，我们分析了急性临床加重期间获得的脑脊液（CSF）细胞，以及随后13个月免疫调节治疗后的疾病缓解。T细胞受体CDR3区长度分布在复发期间显著改变，表明CSF中存在克隆扩增的T细胞。CDR3谱分析是鉴定CNS中疾病相关T细胞的有价值的方法。由爱思唯尔公司出版
Migration of autoreactive T cells into the central nervous system (CNS) compartment is thought to be an important step in the pathogenesis of multiple sclerosis (MS). To follow the evolution of T cell repertoire in the CNS of a patient with relapsing-remitting MS, we analyzed cerebrospinal fluid (CSF) cells obtained during an acute clinical exacerbation, and subsequent disease remission after 13 months of immunomodulatory therapy. T cell receptor CDR3 region length distribution was significantly altered during the relapse, demonstrating the presence of clonally expanded T cells in the CSF. CDR3 spectratyping is a valuable approach to identify disease-associated T cells in the CNS. Published by Elsevier B.V.