The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice
The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice
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DOI：
10.1530/rep-08-0299
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发表时间：
2009-08-01
期刊：
影响因子：
3.8
通讯作者：
Wakayama, Teruhiko
中科院分区：
文献类型：
--
作者：
Van Thuan, Nguyen;Bui, Hong-Thuy;Wakayama, Teruhiko
自第一个利用体细胞核移植技术克隆小鼠Cumulina诞生以来，小鼠的克隆成功率一直很低，绝大多数近交系小鼠从未被克隆过。最近，我们的实验室发现，用组蛋白脱乙酰酶抑制剂（HDACi）-然而，这对近交系无效。在这里，我们首次表明，通过用另一种HDACi，scriptaid处理SCNT胚胎，可以克隆所有重要的近交系小鼠品系，如C57 BL/6，C3 H/He，DBA/2和129/Sv。此外，通过核移植技术的体细胞核重编程和克隆效率的成功显然与克隆小鼠胚胎中新生mRNA的从头合成有关。生殖（2009年）138 309-317
Since the birth of Cumulina, the first mouse clone produced by somatic cell nuclear transfer (SCNT), the success rate of cloning in mice has been extremely low compared with other species and most of the inbred mouse strains have never been cloned. Recently, our laboratory has found that treatment of SCNT mouse embryos with trichostatin A, a histone deacetylase inhibitor (HDACi), improved the full-term development of B6D2F1 mouse clones significantly. However, this was not effective for the inbred strains. Here, we show for the first time that by treating SCNT embryos with another HDACi, scriptaid, all the important inbred mouse strains can be cloned, such as C57BL/6, C3H/He, DBA/2, and 129/Sv. Moreover, the success of somatic nuclear reprogramming and cloning efficiency via nuclear transfer technique is clearly linked to the competent de novo synthesis of nascent mRNA in cloned mouse embryos. Reproduction (2009) 138 309-317