COMPLETE GENOMIC SEQUENCE AND PHYLOGENETIC ANALYSIS OF THE LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV)
COMPLETE GENOMIC SEQUENCE AND PHYLOGENETIC ANALYSIS OF THE LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV)
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DOI：
10.1006/viro.1993.1298
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发表时间：
1993-06-01
期刊：
影响因子：
3.7
通讯作者：
BRINTON, MA
中科院分区：
文献类型：
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作者：
GODENY, EK;CHEN, L;BRINTON, MA
乳酸脱氢酶升高病毒（LDV-C）的神经毒性分离物的RNA基因组明显完整的序列已经确定。LDV-C基因组长度至少为14,222个核苷酸，包含8个开放阅读框（orf）。orf1a编码242.8 kDa的蛋白，位于基因组的5 '端，包含至少两个推测的木瓜蛋白酶样半胱氨酸蛋白酶结构域和一个推测的凝乳胰蛋白酶样丝氨酸蛋白酶结构域。这个ORF以一个UAG停止密码子终止，如果发生-1移码，该密码子可以被绕过。移码区由一个七核苷酸“滑”序列组成，5 ' -UUUAAAC-3 '，后面是一个假定的假结。ORF 1b编码一个155.4 kDa的蛋白，在其n端含有一个依赖RNA的RNA聚合酶和一个由锌指结构域分隔的RNA解旋酶结构域。另一个在冠状病毒和环状病毒中也保守的未知功能域位于ORF 1b产物的c端。预测了乳糜蛋白酶样蛋白酶在orf1a多蛋白和orf1b多蛋白上的3个裂解位点，并初步确定了LDV成熟的非结构蛋白。六个小的，重叠的3 ' ORFs （ORFs 2到7）编码蛋白质，计算大小为25.8,21.6,19.8,23.9,18.9和12.3 kDa。orf7编码病毒粒子核衣壳蛋白Vp-1，而orf6编码非糖基化包膜蛋白Vp2。orf5、4、3和2分别编码糖蛋白，这些糖蛋白可能是病毒粒子包膜蛋白。LDV与马动脉炎病毒、Lelystad病毒（LV）和类人猿出血热病毒密切相关。这四种病毒属于一种新的正链RNA病毒，与冠状病毒和环状病毒有关。
The apparently complete sequence of the RNA genome of the neurovirulent isolate of lactate dehydrogenase-elevating virus (LDV-C) has been determined. The LDV-C genome is at least 14,222 nucleotides in length and contains eight open reading frames (ORFs). ORF 1a, which encodes a protein of 242.8 kDa and is located at the 5′ end of the genome, contains at least two putative papain-like cysteine protease domains, and one putative chymotrypsin-like serine protease domain. This ORF terminates with a UAG stop codon that can be bypassed if a -1 frameshift occurs. The frameshift region consists of a heptanucleotide "slippery" sequence, 5′-UUUAAAC-3′, followed by a putative pseudoknot. ORF 1b encodes a protein of 155.4 kDa containing, in its N-terminal portion, an RNA-dependent RNA polymerase and an RNA helicase domain separated by a Zn finger domain. Another domain of unknown function that is also conserved in coronaviruses and toroviruses is located at the C-terminus of the ORF 1b product. Three cleavage sites in the ORF 1a polyprotein and three in the ORF 1b polyprotein were predicted for the chymotrypsin-like protease and tentatively delimit the mature nonstructural proteins of LDV. Six small, overlapping 3′ ORFs (ORFs 2 through 7) encode proteins with calculated sizes of 25.8, 21.6, 19.8, 23.9, 18.9, and 12.3 kDa. ORF 7 encodes the virion nucleocapsid protein Vp-1, while ORF 6 encodes the nonglycosylated envelope protein Vp2. ORFs 5, 4, 3, and 2 each encode glycoproteins which may be virion envelope proteins. LDV is closely related to equine arteritis virus, Lelystad virus (LV), and simian hemorrhagic fever virus. These four viruses belong to a new group of positive-strand RNA viruses and are related to coronaviruses and toroviruses.