Transcription profiling of Candida albicans cells undergoing the yeast-to-hyphal transition
Transcription profiling of Candida albicans cells undergoing the yeast-to-hyphal transition
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DOI：
10.1091/mbc.e02-05-0272
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发表时间：
2002-10-01
影响因子：
3.3
通讯作者：
Whiteway, M
中科院分区：
文献类型：
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作者：
Nantel, A;Dignard, D;Whiteway, M
致病真菌白色念珠菌响应外部信号从酵母形态转变为菌丝形态的能力与其致病性有关。我们使用玻璃 DNA 微阵列来研究经历这种转变的细胞中 6333 个预测 ORF 的转录谱及其对温度和培养基变化的反应。我们已经鉴定了几个基因，其转录谱与已知毒力因子的转录谱相似，这些毒力因子是通过添加血清和 37°C 生长温度引起的菌丝生长转换来调节的。对这一转变的时程分析确定了在胚管启动之前诱导并在发育过程后期关闭的转录本。删除 Efg1p 和 Cph1p 转录因子的菌株在菌丝形成方面存在缺陷，其对血清和升高温度的反应几乎与在 37°C 无血清条件下生长的野生型菌株的反应相同。因此，Efg1p 和 Cph1p 是激活血清诱导的转录程序所必需的。
The ability of the pathogenic fungus Candida albicans to switch from a yeast to a hyphal morphology in response to external signals is implicated in its pathogenicity. We used glass DNA microarrays to investigate the transcription profiles of 6333 predicted ORFs in cells undergoing this transition and their responses to changes in temperature and culture medium. We have identified several genes whose transcriptional profiles are similar to those of known virulence factors that are modulated by the switch to hyphal growth caused by addition of serum and a 37degreesC growth temperature. Time course analysis of this transition identified transcripts that are induced before germ tube initiation and shut off later in the developmental process. A strain deleted for the Efg1p and Cph1p transcription factors is defective in hyphae formation, and its response to serum and increased temperature is almost identical to the response of a wild-type strain grown at 37degreesC in the absence of serum. Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum.