On the reptation theory of gel electrophoresis
On the reptation theory of gel electrophoresis
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凝胶电泳的蠕动理论探讨
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
J. Noolandi
中科院分区：
文献类型：
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作者：
G. Slater;J. Noolandi
我们介绍了一种新的有偏回归理论，它允许对DNA凝胶电泳进行定性和半定量的研究。我们证明了在典型的电泳实验中，一个很长的带电复动链的端到端向量在电场中的伸展发生在很短的时间内，这导致了依赖于场的迁移率仅与链的大小弱相关，这与在DNA上的实验是一致的。我们还提出了一种实用的技术来避免链的拉伸。最后，预测了许多标度定律，其中大部分是可以通过实验验证的。
We introduce a new biased reptation theory that allows a qualitative and semiquantitative study of DNA gel electrophoresis. We prove that stretching of the end‐to‐end vector of a very long charged reptating chain in an electric field occurs in a short period of time during a typical electrophoresis experiment, and that this leads to a field‐dependent mobility only weaky dependent upon the size of the chain, in agreement with experiments on DNA. We also propose a practical technique to avoid chain stretching. Finally, many scaling laws are predicted, most of them being verifiable by experiment.