Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm.
Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm.
复制标题
氯化镁和多胺可以使小鼠胚胎干细胞分化为滋养外胚层或内胚层。
DOI：
10.1016/j.bbrc.2016.11.108
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发表时间：
2017
影响因子：
3.1
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Tanase;J.;Yokoo;T.;Matsumura;Y.;Kinoshita;M.;Kikuchi;Y.;Suemori;H. and Ohyama;T.
氯化镁和多胺可以稳定DNA和染色质。此外，它们还能在体外诱导核小体聚集和染色质凝集。为了确定提高活细胞核中阳离子浓度的效果，我们将不同浓度的一价、二价和多价阳离子溶液显微注射到小鼠胚胎干细胞(ES)的细胞核中，并追踪它们的命运。在这里，我们表明，细胞核中氯化镁、亚精胺或精胺浓度的升高对小鼠ES细胞产生显著影响，并可将特定群体的细胞分化为滋养外胚层，而滋养外胚层是小鼠ES细胞通常不会产生的谱系，或内胚层。推测细胞分化很可能是由于这些阳离子浓度升高引起染色质的凝聚，包括Oct3/4基因座。
Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensationin vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm. It is hypothesized that the cell differentiation was most probably caused by the condensation of chromatin including theOct3/4locus, which was induced by the elevated concentrations of these cations.