Influence of CYP2A6*4 genotypes on maternal serum cotinine among Chinese nonsmoking pregnant women.
Influence of CYP2A6*4 genotypes on maternal serum cotinine among Chinese nonsmoking pregnant women.
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DOI：
10.1093/ntr/ntt164
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
C. Xie;X. Wen;P. Ding;Tao Liu;Yanhui He;Z. Niu;Jianmiao Lin;S. Yuan;Xiaoling Guo;D. Jia;Wei-Qing Chen
中科院分区：
文献类型：
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作者：
C. Xie;X. Wen;P. Ding;Tao Liu;Yanhui He;Z. Niu;Jianmiao Lin;S. Yuan;Xiaoling Guo;D. Jia;Wei-Qing Chen
血清可替宁是吸烟和二手烟（SHS）暴露的常见生物标志物，但它可能受到尼古丁代谢酶活性的影响。本研究探讨CYP2A6*4基因型对非吸烟孕妇血清可替宁的影响。方法：我们对中国南方545名非吸烟孕妇的数据进行了一项关于SHS暴露和分娩结局的病例对照研究。参与者在医院分娩后立即自我报告其妊娠期间暴露于SHS的状况和持续时间。研究人员采用CYP2A6*4基因型聚合酶链反应和酶联免疫吸附法检测产前产妇血清中可替宁水平。我们根据自我报告的SHS暴露状况和CYP2A6*4基因型对女性进行分层，然后比较她们血清可替宁的中位水平。结果在自我报告非shs暴露的女性中（n = 317）， CYP2A6*1/*1基因型的中位血清可替宁水平为2.83ng/ml， CYP2A6*1/*4基因型为1.39ng/ml， CYP2A6*4/*4基因型为0.77ng/ml。在自我报告SHS暴露的女性中（n = 228）， CYP2A6*1/*1基因型的中位可替宁水平为3.32ng/ml， CYP2A6*1/*4基因型的中位可替宁水平为2.38ng/ml， CYP2A6*4/*4基因型为1.56ng/ml。引人注目的是，自我报告的CYP2A6*1/*4或CYP2A6*4/*4基因型的shs暴露妇女比自我报告的CYP2A6*1/*1基因型的非shs暴露妇女的中位可替宁水平显著降低（而不是更高）（p = 0.012）。结论CYP2A6*4基因型与中国非吸烟孕妇血清可替宁降低有关。测定CYP2A6*4基因型可能有助于提高孕妇和其他非孕妇人群血清可替宁测定SHS暴露的有效性。
INTRODUCTION Serum cotinine is a common biomarker for smoking and secondhand smoke (SHS) exposure, but it can be affected by the activity of nicotine-metabolizing enzymes. This study investigated the influence of CYP2A6*4 genotypes on serum cotinine among nonsmoking pregnant women. METHODS We analyzed the data from 545 Chinese nonsmoking pregnant women in a case-control study on SHS exposure and birth outcomes in southern China. Participants self-reported their status and duration of SHS exposure during pregnancy right after delivery in hospital. Research staff used polymerase chain reaction to genotype CYP2A6*4 and enzyme-linked immunosorbent assay to measure cotinine levels in maternal serum samples collected before delivery. We stratified women by their self-reported SHS exposure status and CYP2A6*4 genotypes and then compared their median levels of serum cotinine. RESULTS Among women who self-reported non-SHS exposure (n = 317), the median serum cotinine levels were 2.83ng/ml for those with CYP2A6*1/*1 genotype, 1.39ng/ml for CYP2A6*1/*4, and 0.77ng/ml for CYP2A6*4/*4, respectively. Among women who self-reported SHS exposure (n = 228), the median cotinine levels were 3.32ng/ml for those with CYP2A6*1/*1 genotype, 2.38ng/ml for CYP2A6*1/*4, and 1.56ng/ml for CYP2A6*4/*4, respectively. Strikingly, self-reported SHS-exposed women with CYP2A6*1/*4 or CYP2A6*4/*4 genotype had significantly lower (rather than higher) median cotinine levels than self-reported non-SHS-exposed women with CYP2A6*1/*1 genotype (p = .012). CONCLUSIONS CYP2A6*4 genotype is associated with lower serum cotinine among Chinese nonsmoking pregnant women. Measuring CYP2A6*4 genotype may help to improve the validity of SHS exposure measurement by serum cotinine in pregnant women and possibly also in other nonpregnant populations.