Residual oil fly ash amplifies allergic cytokines, airway responsiveness, and inflammation in mice
Residual oil fly ash amplifies allergic cytokines, airway responsiveness, and inflammation in mice
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DOI：
10.1164/ajrccm.160.6.9901053
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发表时间：
1999-12-01
影响因子：
24.7
通讯作者：
Costa, DL
中科院分区：
文献类型：
--
作者：
Gavett, SH;Madison, SL;Costa, DL
颗粒物（PM）空气污染可能会增加过敏性哮喘患者的症状严重程度。为了检查PM效应和过敏反应之间可能的相互作用或大于加和反应，利用了过敏性气道疾病的卵清蛋白致敏和激发（OVA）小鼠模型。激发后，向小鼠气管内滴注生理盐水载体或3 mg/kg（类似于60 μ g）的残油飞灰（ROFA），一种富含过渡金属的排放源PM样品。在1、3、8和15 d后检查生理和炎症反应。响应于静脉注射乙酰甲胆碱，ROFA增加总呼吸系统阻力和降低依从性1天后曝光，而OVA的影响持续至少15天后曝光。显着之间的相互作用，主要是在攻击和暴露后8天，特别是在遵守。OVA和ROFA暴露之间的强相互作用（p <0.01）导致支气管肺泡灌洗（BAL）流体嗜酸性粒细胞数目增加8倍（1天）和3倍（3天）。在激发和暴露后1天，在BAL液白细胞介素-4（IL-4）中观察到类似的强相互作用（8倍）。到攻击/暴露后3天，还发现IL-4和IL-5之间存在显着但不太强烈的相互作用。这项研究表明，过敏原的挑战和暴露于排放源颗粒物含有相对较高水平的过渡金属可以相互作用，以增加Th2细胞因子的产生，嗜酸性粒细胞的招聘，和气道高反应性在先前致敏小鼠。
Particulate matter (PM) air pollution may increase symptom severity in allergic asthmatics. To examine possible interaction, or greater than additive responses, between PM effects and allergic responses, an ovalbumin-sensitized and challenged (OVA) mouse model of allergic airways disease was utilized. After challenge, mice were intratracheally instilled with saline vehicle or 3 mg/kg (similar to 60 mu g) residual oil fly ash (ROFA), a transition metal-rich emission source PM sample. Physiological and inflammatory responses were examined 1, 3, 8, and 15 d later. In response to intravenously administered methacholine, ROFA increased total respiratory system resistance and decreased compliance 1 d after exposure, whereas effects of OVA lasted at least 15 d after exposure. Significant interactions between OVA and ROFA were mainly observed 8 d after challenge and exposure, especially with respect to compliance. A strong interaction (p < 0.01) between OVA and ROFA exposure resulted in 8-fold (1 d) and 3-fold (3 d) increases in bronchoalveolar lavage (BAL) fluid eosinophil numbers. A similarly strong interaction (8-fold) was observed in BAL fluid interleukin-4 (IL-4) 1 d after challenge and exposure. Significant though less strong interactions were also found with respect to IL-4 and IL-5 by 3 d postchallenge/exposure. This study shows that allergen challenge and exposure to emission source particulate matter containing relatively high levels of transitions metals can interact to increase Th2 cytokine production, eosinophil recruitment, and airway hyperresponsiveness in previously sensitized mice.