Enhanced Sensitivity of a Galactose Biosensor Fabricated with a Bundle of Conducting Polymer Microtubules
Enhanced Sensitivity of a Galactose Biosensor Fabricated with a Bundle of Conducting Polymer Microtubules
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DOI：
10.1002/elan.201100183
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发表时间：
2011-09-01
期刊：
影响因子：
3
通讯作者：
Son, Yongkeun
中科院分区：
文献类型：
--
作者：
Lee, Kyoung Neung;Lee, Youngkwan;Son, Yongkeun
采用一束导电聚合物管来构建灵敏度更高的生物传感器。与其他固定方法相比，管状结构能够在传感器电极的单位面积上提供更大的酶封装空间。通过将 EDOT 电化学聚合到 ITO 上的模板中来制备束结构。将酶注入小管后，小管上覆盖有 PPDA 通道。传感器对半乳糖的电流响应在 0.1-1 mM 范围内表现出线性行为，平均灵敏度为 6.37 mu A/mMcm(2)。响应时间为30-40秒。将传感器在 4℃ 的磷酸盐缓冲溶液中放置一周后，其灵敏度约为原始灵敏度的 82%。基于S/N=3，检测限为0.01 mM。
A bundle of conducting polymer tubules was employed to build a biosensor of improved sensitivity. The tubule structure was able to provide larger enzyme encapsulating space per unit area of the sensor electrode compared to other immobilizing methods. The bundle structure was prepared by using electrochemical polymerization of EDOT into the template on ITO. After dosing of enzyme into the tubule, it was covered with PPDA channels. The amperometric response of the sensor to galactose showed linear behavior in the range of 0.1-1 mM with an average sensitivity of 6.37 mu A/mMcm(2). The response time was 30-40 s. After keeping the sensor in phosphate buffer solution at 4 degrees C for a week, it showed about 82% of its original sensitivity. The detection limit was 0.01 mM based on S/N=3.