Application of low vacuum scanning electron microscopy for Papanicolaou-stained slides for cytopathology examinations
Application of low vacuum scanning electron microscopy for Papanicolaou-stained slides for cytopathology examinations
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DOI：
10.1093/jmicro/dfw005
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发表时间：
2016-06-01
期刊：
影响因子：
1.8
通讯作者：
Sawabe, Motoji
中科院分区：
文献类型：
--
作者：
Yano, Tetsuya;Soejima, Yurie;Sawabe, Motoji
巴氏染色切片通常使用透射光显微镜进行细胞病理学观察。然而，病理学检查的进展已经产生了对新的诊断工具的需求，因为细胞病理学准备不允许在不损失标本的情况下进行额外的检查，这与组织病理学不同。低真空扫描电子显微镜（LVSEM）可以在没有金属涂层的情况下以超微结构分辨率显示表面形貌。本研究的目的是确定使用LVSEM观察口腔涂片的巴氏染色载玻片所需的条件，而不损失任何标本，并再次使用光学显微镜重新检查相同的载玻片，同时保留细胞病理学信息。
Papanicolaou (Pap)-stained slides are usually observed using a transmitted light microscope for cytopathology. However, progress in pathological examinations has created a need for new diagnostic tools, because cytopathological preparations do not allow additional examinations without a loss of specimen, unlike histopathology. Low-vacuum scanning electron microscopy (LVSEM) can reveal the surface topography at an ultra-structual resolution without metal coating. The aim of this study was to determine the conditions required for observing Pap-stained slides of oral smears using LVSEM without any loss of specimen and to reexamine the same slides again using light microscopy, while preserving the cytopathological information.