Reversal by lymphokines of the age-related hyporesponsiveness to contact sensitization and reduced Ia expression on Langerhans cells.
Reversal by lymphokines of the age-related hyporesponsiveness to contact sensitization and reduced Ia expression on Langerhans cells.
复制标题
淋巴因子逆转与年龄相关的接触致敏反应低下，并减少朗格汉斯细胞上的 Ia 表达。
DOI：
10.1007/bf00585926
复制
发表时间：
1987
影响因子：
3
通讯作者：
Baer,RL
中科院分区：
文献类型：
--
作者：
Belsito,DV;Dersarkissian,RM;Thorbecke,GJ;Baer,RL
用白介素2(IL-2)、干扰素-γ(干扰素-γ)或生理盐水对幼年(3-5月)和老年(16-26月)BALB/c小鼠进行接触敏感性检测。16个月以上的小鼠除了众所周知的T细胞功能受损外，还有Ia+朗格汉斯细胞的数量不足。它们对1-氯-2，4，6-三硝基苯的接触敏化也有受损的细胞介导性反应。老年小鼠的这种低反应性可被老年小鼠的Ness几乎完全逆转，可被IL-2几乎完全逆转，并可被干扰素-γ略微改善。在体内和体外，老年小鼠的皮肤暴露于白介素2或干扰素-γ，导致Ia+朗格汉斯细胞密度增加，接近年轻小鼠的水平。由于干扰素-γ可部分恢复老年动物的接触性超敏反应，而IL-2可完全恢复老年动物的接触性超敏反应，我们认为老年小鼠的低反应性既是由于抗原提呈的朗格汉斯细胞Ia抗原表达缺陷所致，也是由于T细胞功能缺陷所致。
Contact sensitivity responses were evaluated in young adult (3–5 months) and aged (16–26 months) BALB/c mice which were systemically treated with interleukin-2 (IL-2), interferon-γ (IFN-γ) or saline. Mice older than 16 months of age have deficient numbers of Ia+Langerhans cells in addition to their well-known impaired T cell functions. They also have an impaired cell-mediated response to contact sensitization with 1-chloro-2,4,6-trinitrobenzene. This hyporesponsiveness in aged mice can be almost completely reversed by ness in aged mice can be almost completely reversed by IL-2 and is marginally improved by IFN-γ. Exposure of skin from aged mice to interleukin-2 or interferon-γ, both in vivo and invvitro, causes increases in the density of Ia+Langerhans cells to levels approximating those in young mice. Since IFN-γ causes partial restitution while IL-2 fully restores the contact hypersensitivity in aged animals, we conclude that the hyporesponsiveness in aged mice results both from defective Ia antigen expression on the antigen-presenting Langerhans cells and from deficient T cell function.