Expression of HERV-K108 envelope interferes with HIV-1 production
Expression of HERV-K108 envelope interferes with HIV-1 production
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DOI：
10.1016/j.virol.2017.06.004
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发表时间：
2017-09-01
期刊：
影响因子：
3.7
通讯作者：
Mulder, Lubbertus C. F.
中科院分区：
文献类型：
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作者：
Terry, Sandra N.;Manganaro, Lara;Mulder, Lubbertus C. F.
HML-2组的人内源性逆转录病毒(HERV)-K包含编码功能蛋白的全长或近全长元件，分为1型和2型（1型在env基因的5'端有缺失）。由于不同逆转录病毒的蛋白质可以相互作用，我们假设HERV-K包膜（Env）可能影响HIV-1的复制。在这里，我们描述了某些2型HERV-Ks包膜表达对HIV-1产生的负面影响。所有HIV-1和SW毒株均对HERV-K108包膜有不同程度的抑制作用。我们在HERV-K108 Env中发现了四个对抑制1-1W-1产生至关重要的残基。未观察到EGFP表达受到抑制，表明HERV-K Env不影响一般蛋白的产生。这些发现表明，来自某些内源性逆转录病毒的包膜蛋白可以限制外源性慢病毒（如HIV-1）的产生。未来的研究将阐明HERV Envs介导HIV-1抑制的机制。
The human endogenous retroviruses (HERV)-K of the HML-2 group include full-length or near full-length elements encoding functional proteins, and are classified as type-1 or type-2 (type-1 has a deletion in the 5' end of the env gene).Because proteins of different retroviruses can interact, we hypothesized that HERV-K envelope (Env) could influence HIV-1 replication. Here we describe the negative effect of envelope expression of certain type-2 HERV-Ks on HIV-1 production.All HIV-1 and SW strains tested were susceptible to various degrees to inhibition by the HERV-K108 envelope. We identified four residues within HERV-K108 Env as being critical to inhibit 1-1W-1 production. No inhibition was observed on EGFP expression, indicating that HERV-K Env does not affect general protein production.These findings demonstrate that envelope proteins from some endogenous retroviruses can limit production of exogenous lentiviruses such as HIV-1. Future studies will elucidate the mechanism mediating HIV-1 inhibition by HERV Envs.