Effects of Acute and Chronic Trypanosoma cruzi Infection on Pregnancy Outcomes in Mice: Parasite Transmission, Mortality, Delayed Growth, and Organ Damage in Pups.
Effects of Acute and Chronic Trypanosoma cruzi Infection on Pregnancy Outcomes in Mice: Parasite Transmission, Mortality, Delayed Growth, and Organ Damage in Pups.
复制标题
急性和慢性克氏锥虫感染对小鼠妊娠结局的影响：幼鼠的寄生虫传播、死亡率、生长延迟和器官损伤。
DOI：
10.1016/j.ajpath.2022.11.010
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Garg,NishaJ
中科院分区：
文献类型：
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作者：
Rios,LizetteE;Lokugamage,Nandadeva;Garg,NishaJ
Chagas disease is caused byTrypanosoma cruzi. This study aimed to determine the effects ofT. cruziinfection on fertility rate and health of the newborn pups in pregnant mice. Female mice were challenged withT. cruziand mated at 21 days (acute parasitemic phase) or 90 days (chronic parasite persistence phase) after infection. Pups were examined for growth up to 20 days after birth; and parasite burden in brain, heart, skeletal muscle, and intestine was measured by real-time quantitative PCR. The inflammatory infiltrate, necrosis, and fibrosis in pups' heart and brain tissues were evaluated by histology.T. cruziinfection in dams delayed the onset of pregnancy, decreased the fertility rate, and led to vertical transmission of parasite to the pups. Furthermore, infected dams delivered pups that exhibited decreased survival rate, decreased birth weight, and decreased growth rate. Significantly increased inflammation, necrosis, and fibrosis of cardiac and brain tissues were noted in pups born to infected dams. Initial challenge with higher parasite dose had more detrimental effects on fertility rate and pups' health in both acutely and chronically infected dams. In conclusion, mice offer a promising model to evaluate the efficacy of new vaccines and therapeutic drugs in controlling the acute and chronic maternalT. cruziinfection and congenital transmission to newborns, and in improving the fertility rate and pups' health outcomes.
