Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus
Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus
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DOI：
10.1523/jneurosci.4186-04.2005
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发表时间：
2005-03-09
影响因子：
5.3
通讯作者：
Takada, M
中科院分区：
文献类型：
--
作者：
Miyachi, S;Lu, XF;Takada, M
通过狂犬病病毒的逆行跨神经元传输，研究了猕猴前额叶皮质到初级运动皮质(MI)的多突触投射的组织结构。在第一系列实验中，将病毒注射到MI前肢区域，分析了存活时间不同(2-4d)的额叶跨突触标记的时间依赖分布模式。病毒注射两天后，在非初级运动相关区域的尾侧出现神经元标记，已知这些区域直接投射到MI。同时，运动丘脑也含有标记神经元。第3天，皮质标记延伸至吻侧运动相关区和弓前8区，并在苍白球内侧核和小脑核内可见大量标记神经元。注射后4d，前额叶、壳核和小脑皮质广泛出现神经元标记。在第二系列实验中，将病毒注射到MI后肢区域，并比较第4天前额叶标记的分布模式与前肢注射情况。后肢注射组前额叶各区标记神经元数量明显少于前肢注射组。腹侧46区是前肢区域标记最密集的区域，而前额叶区域仅观察到稀疏的后肢区域标记。本研究结果提示腹侧区46在前肢运动认知控制中的重要性。
The organization of multisynaptic projections from the prefrontal cortex to the primary motor cortex (MI) was examined in macaque monkeys by retrograde transneuronal transport of rabies virus. In the first series of experiments, the virus was injected into the MI forelimb region, and the time-dependent distribution patterns of transsynaptic labeling were analyzed in the frontal lobe with various survivals (2-4 d). Two days after the viral injection, neuronal labeling emerged in the caudal aspects of the nonprimary motor-related areas that are known to project to the MI directly. At the same time, the motor thalamus contained labeled neurons. On the third day, cortical labeling extended into the rostral motor-related areas and, also, prearcuate area 8. Moreover, a number of labeled neurons were located in the internal pallidum and the cerebellar nuclei. At the 4 d postinjection period, neuronal labeling occurred widely in prefrontal areas as well as in the putamen and the cerebellar cortex. In the second series of experiments, the viral injection was made into the MI hindlimb region, and the distribution pattern of prefrontal labeling on the fourth day was compared with that in the forelimb-injection case. The labeled neurons in each prefrontal area were much fewer in the hindlimb-injection case than in the forelimb-injection case. Whereas ventral area 46 was most densely labeled from the forelimb region, only sparse labeling from the hindlimb region was observed in this prefrontal area. The present results suggest the importance of ventral area 46 in the cognitive control of forelimb movements.