Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana
Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana
复制标题
拟南芥中抑制植物病毒系统运动的类压电蛋白的遗传分析
DOI：
10.1038/s41598-019-39436-3
复制
发表时间：
2019-02-28
影响因子：
4.6
通讯作者：
Wang, Xian-Bing
中科院分区：
文献类型：
--
作者：
Zhang, Zhen;Tong, Xin;Wang, Xian-Bing
植物病毒作为专性胞内病原菌，必须利用寄主的胞间连丝和韧皮部脉管系统进行局部和远距离运输，才能在植物体内建立系统性感染。与病毒的局部运输相比，控制病毒系统运输的分子机制和相关宿主基因还远未被理解。在这里，我们进行了正向遗传筛选，以确定拟南芥突变体的敏感性增强黄瓜花叶病毒（CMV-2aT）的2b缺失突变体（2b）。我们发现，一个未表征的压电蛋白（AtPiezo），一个直系同源物的动物压电蛋白与机械敏感（MS）阳离子通道活动，是需要抑制系统感染的CMV-2aT_2b和芜菁花叶病毒标记的绿色荧光蛋白（GFP）（TuMV-GFP）。AtPiezo是由病毒感染引起的，特别是在莲座叶的叶柄中。因此，我们首次证明了植物中Piezo蛋白的生物学功能，这可能代表了一种常见的抗病毒策略，因为许多单子叶植物和双子叶植物物种都有一个单一的Piezo直系同源物。
As obligate intracellular phytopathogens, plant viruses must take advantage of hosts plasmodesmata and phloem vasculature for their local and long-distance transports to establish systemic infection in plants. In contrast to well-studied virus local transports, molecular mechanisms and related host genes governing virus systemic trafficking are far from being understood. Here, we performed a forward genetic screening to identify Arabidopsis thaliana mutants with enhanced susceptibility to a 2b-deleted mutant of cucumber mosaic virus (CMV-2aT∆2b). We found that an uncharacterized Piezo protein (AtPiezo), an ortholog of animal Piezo proteins with mechanosensitive (MS) cation channel activities, was required for inhibiting systemic infection of CMV-2aT∆2b and turnip mosaic virus tagged a green fluorescent protein (GFP) (TuMV-GFP). AtPiezo is induced by virus infection, especially in the petioles of rosette leaves. Thus, we for the first time demonstrate the biological function of Piezo proteins in plants, which might represent a common antiviral strategy because many monocot and dicot plant species have a single Piezo ortholog.