Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation
Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation
复制标题
使用同步辐射对大鼠脑血管结构进行超高分辨率 3D 数字化成像
DOI：
10.1038/srep14982
复制
发表时间：
2015-10-07
影响因子：
4.6
通讯作者：
Xiao, Bo
中科院分区：
文献类型：
--
作者：
Zhang, Meng-Qi;Zhou, Luo;Xiao, Bo
血管构筑是大脑发育和生理学的一个基本方面。然而，现有的成像工具不适合于对具有重要功能的三维(3D)血管微结构进行非破坏性的大脑标测。为了解决这一问题，我们开发了一种基于同步辐射相衬成像的超高分辨率3D数字化大鼠脑血管构筑图，像素尺寸为5.92 μm，该方法提供了微米级的系统和详细的脑血管解剖图像，不需要任何造影剂。从定性和定量的角度，目前的3D数据为整个啮齿动物大脑的空间血管网络提供了相当大的洞察力，特别是在功能上重要的感兴趣区域，如海马体、额叶前皮质和纹状体。我们将这些结果扩展到基于同步加速器的虚拟显微内窥镜检查，从而揭示了目标血管的3D轨迹。用于脑微血管系统可视化的SR-PCI方法在生命科学中具有广泛的应用前景，包括在神经发育和血管疾病的实验模型中的3D显微成像。
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.